Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences
Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences
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DOI：
10.1163/156854102765216731
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发表时间：
2003-01-01
期刊：
影响因子：
1.2
通讯作者：
Moens, M
中科院分区：
文献类型：
--
作者：
Maafi, ZT;Subbotin, SA;Moens, M
从伊朗不同地区收集的45个群体的囊形成线虫的RFLP和ITS-rDNA序列进行了分析，并确定为21种的代表。八种酶产生RFLP的所有研究人群。ITS区的RFLP图谱和序列与已发表的数据进行比较，证实了Heterodera avenue，H. filipjevi，H. glycines，H. hordecalis，H. latipons，H. schachtii和H.伊朗的三叶草。RFLP图谱和ITS序列分析表明，H. elachista，H. turcomanica，H. mothi和C.仙人掌是本研究中首次获得的。Heterodera humuli，H. goettingiana、黑腹叶蝉H. fici，H. elachista，H. turcomanica和Cactodera cacti为首次在伊朗发现。这些结果与种群的形态学和形态计量学鉴定相一致。有几个种群在物种水平上没有得到鉴定，被认为是胞囊线虫;其中一些可能对应于新物种。从伊朗囊形成线虫和36个已知序列的21个新的序列用于系统发育分析。孢囊形成线虫形成了几个分支对应于他们的形态特征。Heterodera mothi和H. elachista与其他Cybergium类群的物种和H. turcomanica与Humuli群形成了一个中等到高度支持的分支。
RFLP and sequences of ITS-rDNA of 45 populations of cyst-forming nematodes collected from different parts of Iran were analysed and identified as representatives of 21 species. Eight enzymes generated RFLP for all studied populations. Comparison of RFLP profiles and sequences of the ITS regions with published data confirmed the presence of Heterodera avenue, H. filipjevi, H. glycines, H. hordecalis, H. latipons, H. schachtii and H. trifolii in Iran. RFLP patterns and ITS sequences for H. elachista, H. turcomanica, H. mothi and C. cacti were obtained for the first time in this study. Heterodera humuli, H. goettingiana, H. fici, H. elachista, H. turcomanica and Cactodera cacti are recorded for the first time in Iran. These results correspond with morphological and morphometric identification of the populations. Several populations were not identified at the species level and are attributed to Heterodera sp.; some of these may correspond to new species. Twenty-one new sequences from Iranian cyst-forming nematodes and 36 known sequences were used for the phylogenetic analyses. The cyst-forming nematodes formed several clades corresponding to their morphological features. Heterodera mothi and H. elachista clustered with high support with other Cyperi group species and H. turcomanica formed a moderately to highly supported clade with the Humuli group.