Assessment of different bariatric surgeries in the treatment of obesity and insulin resistance in mice.
Assessment of different bariatric surgeries in the treatment of obesity and insulin resistance in mice.
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DOI：
10.1097/sla.0b013e3182197035
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发表时间：
2011-07
影响因子：
9
通讯作者：
Abumrad NN
中科院分区：
文献类型：
--
作者：
Yin DP;Gao Q;Ma LL;Yan W;Williams PE;McGuinness OP;Wasserman DH;Abumrad NN
评估不同减肥手术对高脂饮食诱导肥胖（DIO）小鼠肥胖和胰岛素抵抗的治疗效果。减肥手术目前被认为是治疗病态肥胖及其合并症的最有效的方法，然而，其机制的系统研究仍然缺乏。我们在DIO小鼠中开发了减肥手术模型，包括胃束带术、袖状胃切除术、Roux-en-Y胃旁路术（RYGB）、改良RYGB（mRYGB）和胆胰分流术（BPD）。检查体重、体脂和瘦体重、肝脏脂肪变性、葡萄糖耐量和胰腺β细胞功能。与假手术小鼠相比，所有减肥手术均导致体重显著减轻，体脂减少，短期（4周）葡萄糖耐量改善。在减肥手术模型中，袖状胃切除术和mRYGB具有较高的成功率和较低的死亡率，分别代表可靠的限制性和胃肠道（GI）旁路小鼠减肥手术模型。从长远来看，胃肠道旁路手术比限制性手术产生了更深刻的体重减轻，葡萄糖耐量和肝脏脂肪变性的显着改善。DIO小鼠具有增加的胰岛素启动子活性，表明胰腺β细胞的过度活化，其由mRYGB程序调节。与限制性手术相比，胃肠道旁路手术在减肥手术后显示出更严重的营养不良症状。限制性手术和胃肠道旁路手术对体重减轻、脂肪组成、肝脏脂肪变性和葡萄糖耐量均有积极作用;然而，从长远来看，胃肠道旁路手术比限制性手术显示出更好的结果。
To assess the effects of different bariatric surgical procedures on the treatment of obesity and insulin resistance in high fat diet-induced obese (DIO) mice. Bariatric surgery is currently considered the most effective treatment for morbid obesity and its comorbidities; however, a systematic study of their mechanisms is still lacking. We developed bariatric surgery models, including gastric banding, sleeve gastrectomy, Roux-en-Y gastric bypass (RYGB), modified RYGB (mRYGB) and biliopancreatic diversion (BPD), in DIO mice. Body weight, body fat and lean mass, liver steatosis, glucose tolerance and pancreatic beta cell function were examined. All bariatric surgeries resulted in significant weight loss, reduced body fat and improved glucose tolerance in the short term (4 weeks), compared to mice with sham surgery. Of the bariatric surgery models, sleeve gastrectomy and mRYGB had higher success rates and lower mortalities and represent reliable restrictive and gastrointestinal (GI) bypass mouse bariatric surgery models, respectively. In the long term, the GI bypass procedure produced more profound weight loss, significant improvement of glucose tolerance and liver steatosis than the restrictive procedure. DIO mice had increased insulin promoter activity, suggesting over-activation of pancreatic beta cells, which was regulated by the mRYGB procedure. Compared to the restrictive procedure, the GI bypass procedure showed more severe symptoms of malnutrition following bariatric surgery. Both restrictive and GI bypass procedures provide positive effects on weight loss, fat composition, liver steatosis and glucose tolerance; however, in the long term, the GI bypass shows better results than restrictive procedures.