TOXOPLASMA-GONDII COMPRISES 3 CLONAL LINEAGES - CORRELATION OF PARASITE GENOTYPE WITH HUMAN-DISEASE
TOXOPLASMA-GONDII COMPRISES 3 CLONAL LINEAGES - CORRELATION OF PARASITE GENOTYPE WITH HUMAN-DISEASE
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DOI：
10.1093/infdis/172.6.1561
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发表时间：
1995-12-01
影响因子：
6.4
通讯作者：
SIBLEY, LD
中科院分区：
文献类型：
--
作者：
HOWE, DK;SIBLEY, LD
应用多位点限制性片段长度多态性分析方法，对106株人源和动物源弓形虫分离株的6个位点进行了分析。广泛混合的基因型仅在4个菌株中表现出来，这表明，虽然不是单独的物种，但3个谱系之间的有性重组在自然群体中是非常罕见的，T。弓形虫是人类亚临床感染的主要原因，也是导致免疫功能低下患者严重疾病的重要条件致病菌。虽然来自所有3个谱系的菌株均分离自人类，但大多数人类弓形虫病病例与II型基因型菌株相关。特异性克隆谱系与人类弓形虫病的相关性对于疫苗、药物治疗和诊断方案的开发具有重要意义。
The population genetic structure of Toxoplasma gondii was determined by multilocus restriction fragment length polymorphism analysis at 6 loci in 106 independent isolates from humans and animals, Phylogenetic and statistical analyses indicated a highly unusual population structure consisting of 3 widespread clonal lineages. Extensively mixed genotypes were only apparent in 4 strains, which indicated that, while not separate species, sexual recombination between the 3 lineages is exceedingly rare in natural populations, T. gondii is a major cause of subclinical human infection and an important opportunistic pathogen that causes severe disease in immunocompromised patients. While strains from all 3 lineages were isolated from humans, the majority of human toxoplasmosis cases were associated with strains of a type II genotype. The correlation of specific clonal lineages with human toxoplasmosis has important implications for development of vaccines, drug treatments, and diagnostic protocols.