Rapid Identification of Cryptococcus neoformans and Cryptococcus gattii by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
Rapid Identification of Cryptococcus neoformans and Cryptococcus gattii by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry
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DOI：
10.1128/jcm.00651-11
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发表时间：
2011-08-01
影响因子：
9.4
通讯作者：
Zhang, Sean X.
中科院分区：
文献类型：
--
作者：
McTaggart, Lisa R.;Lei, Eric;Zhang, Sean X.
与DNA序列分析相比，基质辅助激光解吸电离飞行时间质谱法（MALDI-TOF MS）对隐球菌种类的鉴定准确率达到100%，区分出了值得注意的病原体新型隐球菌和C. gatti。通过补充商业光谱库的额外条目来解释亚种的可变性，极大地增强了鉴定。
Compared to DNA sequence analysis, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) correctly identified 100% of Cryptococcus species, distinguishing the notable pathogens Cryptococcus neoformans and C. gattii. Identification was greatly enhanced by supplementing a commercial spectral library with additional entries to account for subspecies variability.