Antibiotics as a selective driver for conjugation dynamics.
Antibiotics as a selective driver for conjugation dynamics.
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DOI：
10.1038/nmicrobiol.2016.44
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发表时间：
2016-04-11
影响因子：
28.3
通讯作者：
You, Lingchong
中科院分区：
文献类型：
--
作者：
Lopatkin, Allison J.;Huang, Shuqiang;Smith, Robert P.;Srimani, Jaydeep K.;Sysoeva, Tatyana A.;Bewick, Sharon;Karig, David K.;You, Lingchong
一般认为抗生素可以促进水平基因转移(HGT)。然而，由于各种混杂因素使先前研究的解释复杂化，抗生素调节HGT的机制仍然知之甚少。特别是，目前尚不清楚抗生素是直接调节HGT的效率，还是在HGT发生后作为一种选择力量来调节种群动态，或者两者兼而有之。在这里，我们通过量化在抗生素介导的选择存在和不存在的情况下的结合动力学来解决这个问题。令人惊讶的是，我们发现来自最广泛使用的类别的亚致死浓度的抗生素并没有显著提高偶联效率。相反，我们的模型和实验结果表明，接合动力学是由抗生素介导的选择决定的，这既可以促进接合动力学，也可以抑制接合动力学。我们的发现表明，抗生素对促进HGT的贡献可能被高估了。这些发现对设计有效的抗生素治疗方案和评估抗生素使用的风险具有重要意义。
It is generally assumed that antibiotics can promote horizontal gene transfer (HGT). However, because of a variety of confounding factors that complicate the interpretation of previous studies, the mechanisms by which antibiotics modulate HGT remain poorly understood. In particular, it is unclear whether antibiotics directly regulate the efficiency of HGT, serve as a selection force to modulate population dynamics after HGT has occurred, or both. Here, we address this question by quantifying conjugation dynamics in the presence and absence of antibiotic-mediated selection. Surprisingly, we find that sub-lethal concentrations of antibiotics from the most widely used classes do not significantly increase the conjugation efficiency. Instead, our modeling and experimental results demonstrate that conjugation dynamics are dictated by antibiotic-mediated selection, which can both promote and suppress conjugation dynamics. Our findings suggest that the contribution of antibiotics to the promotion of HGT may have been overestimated. These findings have implications for designing effective antibiotic treatment protocols and for assessing the risks of antibiotic use.
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影响因子：
64.8
作者：
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通讯作者：
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影响因子：
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DOI：
10.1073/pnas.0806786105
发表时间：
2008-11-18
影响因子：
11.1
作者：
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通讯作者：
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