Effects of staphylococci on cytokine production from human keratinocytes
Effects of staphylococci on cytokine production from human keratinocytes
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DOI：
10.1046/j.1365-2133.2003.05017.x
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发表时间：
2003-01-01
影响因子：
10.3
通讯作者：
Hasegawa, A
中科院分区：
文献类型：
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作者：
Sasaki, T;Kano, R;Hasegawa, A
背景葡萄球菌皮肤感染的特征是表皮内大量中性粒细胞浸润;然而，在皮肤感染葡萄球菌期间，中性粒细胞表皮浸润的精确机制还没有很好地理解，并且调节中性粒细胞募集的因素尚未确定。目的我们研究了葡萄球菌对角质形成细胞产生细胞因子的影响，方法用热灭活的金黄色葡萄球菌（Staphylococcus aureus）、S.表皮葡萄球菌和表皮葡萄球菌。结果用酶联免疫吸附试验检测到IL-6和IL-8，但未检测到IL-1 β、单核细胞趋化蛋白-1和肿瘤坏死因子-α。用灭活的葡萄球菌刺激角质形成细胞1、3、6、10和24 h后，逆转录聚合酶链反应检测到IL-6和IL-8 mRNA的表达。结论这些结果可以解释皮肤葡萄球菌感染中中性粒细胞的表皮浸润，提示细胞因子的分析可能为皮肤葡萄球菌感染的发病机制提供有价值的信息。
Background Staphylococcus skin infection is characterized by the infiltration of numerous neutrophils within the epidermis; however, the precise mechanism of epidermal infiltration of neutrophils during skin infection with staphylococci is not well understood and the factors regulating the neutrophil recruitment are yet to be determined.Objectives We investigated the effects of staphylococci on cytokine production from keratinocytes, specifically to elucidate the mechanisms of neutrophil infiltration within the epidermis in cutaneous microbial infection.Methods Cytokine production from human keratinocytes was examined after stimulation with heat-killed Staphylococcus aureus, S. epidermidis and S. intermedius.Results Interleukin (IL)-6 and IL-8 were detected in the culture supernatants by enzyme-linked immunosorbent assay but IL-1beta, monocyte chemotactic protein-1 and tumour necrosis factor-alpha were not. IL-6 and IL-8 mRNAs were also confirmed by reverse transcription-polymerase chain reaction in the keratinocytes stimulated with killed staphylococci for 1, 3, 6, 10 and 24 h.Conclusions These results could explain the epidermal infiltration of neutrophils in cutaneous infection with staphylococci, suggesting that the analysis of cytokines might add valuable information for the pathogenesis of cutaneous infection with Staphylococcus species.