Hypoxia and reoxygenation augment bone-resorbing factor production from human periodontal ligament cells
Hypoxia and reoxygenation augment bone-resorbing factor production from human periodontal ligament cells
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DOI：
10.1902/jop.2007.060519
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发表时间：
2007-09-01
影响因子：
4.3
通讯作者：
Shin, Kitetsu
中科院分区：
文献类型：
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作者：
Motohira, Hitoshi;Hayashi, Joichiro;Shin, Kitetsu
背景：咬合创伤和吸烟引起的缺氧可能与牙周炎的骨吸收有关。在本研究中，研究了缺氧和复氧对培养的人牙周膜（PDL）细胞产生骨吸收因子的影响。方法：人牙周膜细胞在1％O-2（缺氧）、20％O-2（正常氧张力[常氧]）或1％至20％的氧气浓度（复氧）中培养。通过酶联免疫吸附测定测定细胞培养上清液中骨吸收因子的浓度，即血管内皮生长因子（VEGF）、白细胞介素（IL）-6和-1β、肿瘤坏死因子-α（TNF-α）和前列腺素E-2（PGE（2））。采用逆转录聚合酶链式反应检测相应mRNA的表达。结果：与常氧组相比，缺氧组胞外VEGF和IL-6浓度显着升高，相应mRNA表达也较高。仅在缺氧组中观察到IL-1β的蛋白产生和mRNA表达。两组样品中均未检测到 TNF-α 和 PGE(2)，但检测到了环氧合酶 2 mRNA。然而，再氧合后检测到PGE(2)。此外，复氧后细胞外浓度和mRNA水平也趋于增加VEGF和IL-6和-1β的产生。结论：缺氧和复氧可能刺激PDL产生VEGF、IL-6和-1β以及PGE(2)，从而导致牙周炎牙槽骨的吸收。
Background: Oxygen deficiency caused by occlusal trauma and smoking may be associated with bone resorption in periodontitis. In the present study, the effects of hypoxia and reoxygenation on the production of bone-resorbing factors by cultured human periodontal ligament (PDL) cells were examined.Methods: Human PDL cells were cultured in 1 % O-2 (hypoxia), 20% O-2 (normal oxygen tension [normoxia]), or an oxygen concentration that went from 1 % to 20% (reoxygenation). The concentrations of bone-resorbing factors, i.e., vascular endothelial growth factor (VEGF), interleukin (IL)-6 and -1 beta, tumor necrosis factor-alpha (TNF-alpha), and prostaglandin E-2 (PGE(2)), in the cell culture supernatants were determined by enzyme-linked immunosorbent assay. Expression of the corresponding mRNAs was detected by reverse transcription-polymerase chain reaction.Results: Significantly higher extracellular concentrations of VEGF and IL-6 were detected along with greater corresponding mRNA expression in the hypoxia group compared to the normoxia group. The protein production and mRNA expression of IL-1 beta were observed only in the hypoxia group. Neither TNF-alpha nor PGE(2) was detectable in samples from either group, whereas cyclooxygenase-2 mRNA was detected. However, PGE(2) was detected after reoxygenation. Furthermore, VEGF and IL-6 and -1 beta production also tended to increase in extracellular concentration and mRNA level after reoxygenation.Conclusion: Hypoxia and reoxygenation may stimulate the PDL to produce VEGF, IL-6 and -1 beta, and PGE(2), which could result in the resorption of alveolar bone in periodontitis.