Construction of a Supramolecular Forster Resonance Energy Transfer System and Its Application Based on the Interaction between Cy3-Labeled Melittin and Phosphocholine Encapsulated Quantum Dots
Construction of a Supramolecular Forster Resonance Energy Transfer System and Its Application Based on the Interaction between Cy3-Labeled Melittin and Phosphocholine Encapsulated Quantum Dots
复制标题
基于Cy3标记蜂毒肽与磷酸胆碱封装量子点相互作用的超分子福斯特共振能量转移体系的构建及其应用
DOI：
10.1021/am3000984
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发表时间：
2012-03-01
影响因子：
9.5
通讯作者：
Wu, Yuqing
中科院分区：
文献类型：
--
作者：
Dang, Yong-Qiang;Li, Hong-Wei;Wu, Yuqing
半导体量子点具有独特的光学性质，已被应用于构建生物复合物。利用量子点作为供体，Forster共振能量转移（FRET）系统可以被开发并应用于生物成像和传感，并且已经报道了各种构建策略。为了提供一种新的可行的方法，我们介绍了一个协议，有两条路线，构建一个超分子FRET系统的基础上的蜂毒肽和磷酸胆碱之间的高亲和力的相互作用。蜂毒肽在水性环境中以无规卷曲结构存在，但当插入天然或人工膜中时将采用弯曲的螺旋。这种特异性和高亲和力的蛋白质膜相互作用使得构建基于量子点的FRET系统成为可能。利用蛋白质膜相互作用构建基于量子点的FRET系统的策略，可以通过距离依赖的FRET研究蛋白质膜相互作用，并进一步研究胰蛋白酶的蛋白水解。由于在真实的生活中存在着各种蛋白质膜相互作用，该系统具有扩展到其他相关系统的潜力。
Due to possessing unique optical properties, semiconductor quantum dots (QDs) have been applied to construct bioconjugates. Using QDs as donors, the Forster resonance energy transfer (FRET) system can be developed and applied to biological imaging and sensing, and various construction strategies have been reported. To provide a new practicable method, we introduce a protocol with two routes to construct a supramolecular FRET system based on the high-affinity interaction between melittin and phosphocholine. Melittin exists with a random coil structure in aqueous environments but will adopt a bent helix when inserted into natural or artificial membranes. Such specific and high affinity protein membrane interaction makes it possible to construct a QDs-based FRET system. The strategy applying protein membrane interaction to construct a QDs-based FRET system can be applied to the investigation on the protein membrane interaction through distance-depended FRET and further proteolysis of trypsin. Because of the existence of various protein membrane interactions in real life, the system has the potential to be expanded to other related systems.