Dynactin is essential for growth cone advance
Dynactin is essential for growth cone advance
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DOI：
10.1016/j.bbrc.2008.05.008
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发表时间：
2008-08-01
影响因子：
3.1
通讯作者：
Kuwano, Ryozo
中科院分区：
文献类型：
--
作者：
Abe, Takako K.;Honda, Takao;Kuwano, Ryozo
动力蛋白是一种多亚基复合物，是微管运动细胞质动力蛋白的关键辅因子。我们之前在神经生长锥中发现了动力蛋白。然而，dynactin在生长锥中的作用尚不清楚。在这里，我们表明生长锥中的动态蛋白是生长锥不断向前运动所必需的。发色团辅助激光失活（CALI）生长锥内的动态蛋白亚基动态蛋白可显著降低生长锥的前进速度。动力蛋白的CALI在体外将另一个动力蛋白亚基p150（glue）从动力蛋白中分离出来。这些结果表明，动力蛋白，特别是动力蛋白与p150（glue）的相互作用，在生长锥的发育过程中起着重要的作用。(C) 2008爱思唯尔公司版权所有。
Dynactin is a multi-subunit complex that serves as a critical cofactor of the microtubule motor cytoplasmic dynein. We previously identified dynactin in the nerve growth cone. However, the function of dynactin in the growth cone is still unclear. Here we show that dynactin in the growth cone is required for constant forward movement of the growth cone. Chromophore-assisted laser inactivation (CALI) of dynamitin, a dynactin subunit, within the growth cone markedly decreases the rate of growth cone advance. CALI of dynamitin in vitro dissociates another dynactin subunit, p150(Glued), from dynamitin. These results indicate that dynactin, especially the interaction between dynamitin and p150(Glued), plays an essential role in growth cone advance. (C) 2008 Elsevier Inc. All rights reserved.