Insights Into the Early Evolution of SOX Genes From Expression Analyses in a Ctenophore
Insights Into the Early Evolution of SOX Genes From Expression Analyses in a Ctenophore
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DOI：
10.1002/jez.b.21244
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发表时间：
2008-12-15
影响因子：
2.2
通讯作者：
Manuel, Michael
中科院分区：
文献类型：
--
作者：
Jager, Muriel;Queinnec, Eric;Manuel, Michael
SOX基因编码的转录因子在双侧动物的各种发育过程中起作用，例如在各种情况下，干细胞维持和细胞类型的规范和分化的控制，特别是在发育中的神经系统中。为了深入了解这个重要的发育调控家族的早期进化，我们研究了一个B亚群，两个E亚群，一个F亚群和两个不同的SOX基因在cydippid幼虫和栉水母Pleurobrachia pileus成虫中的表达。两个未分类的SOX （PpiSOX2/12）的转录本在雌性生殖系和各种假定的体细胞干细胞/未分化祖细胞群体中被检测到。其余基因在纤毛上皮细胞中具有空间限制性表达模式，特别是在神经感觉区域。这些数据与SOX蛋白在干细胞维持、细胞分化和规范的控制方面的古老参与是一致的，特别是在神经感觉上皮内。此外，这些结果突出了栉水母解剖结构的复杂性，并表明SOX在进化过程中通过多基因合作选择，在形成独特的栉水母体计划中发挥了重要作用。J. Exp. Zool。（Mol. Dev. evolution .）310 b: 650 - 667, 2008。(c) 2008 Wiley-Liss, Inc。
SOX genes encode transcription factors acting in various developmental processes in bilaterian animals, such as stem cell maintenance and the control of specification and differentiation of cell types in a variety of contexts, notably in the developing nervous system. To gain insights into the early evolution of this important family of developmental regulators, we investigated the expression of one subgroup B, two subgroup E, one subgroup F and two divergent SOX genes in the cydippid larva and in the adult of the ctenophore Pleurobrachia pileus. Transcripts of the two unclassified SOX (PpiSOX2/12) were detected in the female germ line and in various populations of putative somatic stem cells/undifferentiated progenitors. The remaining genes had spatially restricted expression patterns in ciliated epithelial cells, notably within neuro-sensory territories. These data are compatible with an ancient involvement of SOX proteins in controlling aspects of stem cell maintenance, cellular differentiation and specification, notably within neuro-sensory epithelia. In addition, the results highlight the complexity of the ctenophore anatomy and suggest that the SOX played an important role in the elaboration of the unique ctenophore body plan during evolution, through multiple gene co-option. J. Exp. Zool. (Mol. Dev. Evol.) 310B:650-667, 2008. (c) 2008 Wiley-Liss, Inc.