Cell-specific expression and regulation of soluble guanylyl cyclase alpha 1 and beta 1 subunits in the rat ovary.
Cell-specific expression and regulation of soluble guanylyl cyclase alpha 1 and beta 1 subunits in the rat ovary.
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发表时间：
2004
影响因子：
3.6
通讯作者：
F. Shi;R. Stewart;E. Perez;J. Chen;P. Lapolt
中科院分区：
文献类型：
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作者：
F. Shi;R. Stewart;E. Perez;J. Chen;P. Lapolt
一氧化氮(NO)和一氧化碳(CO)激活可溶性鸟苷酸环化酶(SGC)，产生cGMP。最近的研究表明，NO和cGMP影响卵巢功能。然而，关于sGC的卵巢表达的信息很少。本研究检测了sGCα(1)和β(1)亚单位蛋白在出生后发育、促性腺激素诱导的卵泡生长、排卵和黄体化以及培养的大鼠颗粒细胞中的水平。出生后第5天，原始卵泡和初级卵泡的颗粒细胞中sGCα(1)亚基免疫反应较强，第10天和19天较大卵泡的颗粒细胞中sGCα(1)免疫反应较弱。发育中的卵泡膜细胞也呈中等水平的sGCα(1)免疫反应，而基质细胞则不表达。促性腺激素处理的幼鼠，原始卵泡和初级卵泡的颗粒细胞中sGCα(1)亚单位的染色强度相似，但在小腔卵泡的颗粒细胞中染色较弱，而在大的有腔卵泡和排卵前卵泡的颗粒细胞中检测不到这种染色。排卵后，黄体呈中等sGCα(1)免疫反应。SGCβ(1)的卵巢定位和表达模式相似，表明sGC亚单位受调控的共表达。免疫印迹分析显示，促性腺激素治疗期间，卵巢总sGCα(1)和β(1)亚单位蛋白水平没有变化。同样，在培养的颗粒细胞中，FSH对sGC亚单位蛋白水平没有明显影响。这些发现表明sGC在卵巢中的表达受到调节，具有细胞特异性，并与cGMP在调节卵巢功能中的作用一致。
Soluble guanylyl cyclase (sGC) is activated by nitric oxide (NO) and carbon monoxide, resulting in cGMP production. Recent studies indicate that NO and cGMP influence ovarian functions. However, little information is available regarding the ovarian expression of sGC. The present study examined sGC alpha(1) and beta(1) subunit protein levels in the ovary during postnatal development, gonadotropin-induced follicle growth, ovulation, and luteinization as well as in cultured rat granulosa cells. In postnatal rats, sGC alpha(1) subunit immunoreactivity was high in granulosa cells of primordial and primary follicles on Day 5 but low in granulosa cells of larger follicles on Days 10 and 19. Theca cells of developing follicles, but not stromal cells, also demonstrated moderate sGC alpha(1) immunoreactivity. In gonadotropin- treated immature rats, intense sGC alpha(1) subunit staining was similarly observed in granulosa cells of primordial and primary follicles, but such staining was low in granulosa cells of small antral follicles and undetectable in granulosa cells of large antral and preovulatory follicles. Following ovulation, corpora lutea expressed moderate sGC alpha(1) immunoreactivity. Similar ovarian localization and expression patterns were seen for sGC beta(1), indicating regulated coexpression of sGC subunits. Immunoblot analysis revealed no change in total ovarian sGC alpha(1) and beta(1) subunit protein levels during gonadotropin treatment. Similarly, no effect of FSH on sGC subunit protein levels was apparent in cultured granulosa cells. These findings indicate regulated, cell- specific patterns of sGC expression in the ovary and are consistent with roles for cGMP in modulating ovarian functions.