Bicarbonate and its role in mammalian sperm function
Bicarbonate and its role in mammalian sperm function
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DOI：
10.1016/j.anireprosci.2004.04.030
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发表时间：
2004-07-01
影响因子：
2.2
通讯作者：
van Gestel, RA
中科院分区：
文献类型：
--
作者：
Gadella, BM;van Gestel, RA
本文论述了碳酸氢盐诱导的信号通路对质膜脂质组织和下游蛋白信号的影响，以及它们在精卵相互作用中的作用。它还提供了结果的概述，表明碳酸氢盐反应与细胞死亡或凋亡无关。所提供的信息表明，只有那些在附睾中功能上完全成熟的精子细胞对碳酸氢盐敏感，而未成熟的精子对这种生理挑战没有反应。因此，在研究精子获能时，有选择地分析反应性精子亚群是重要的。此外，碳酸氢盐诱导的信号传导反应在不同的精子结构内不同（例如尾部与头部）。因此，在精子获能研究中，解剖精子结构和信号传导区域值得更多的关注。所讨论的信息是从各种哺乳动物物种中获得的，但在大多数真兽目物种中，基本的碳酸氢盐介导的精子反应是相似的，尽管有些物种与物种之间存在差异（最明显的是动力学而不是事件的顺序）。本文的目的是提供碳酸氢盐介导的精子获能的比较实验概述，希望这些信息将导致更好地了解复杂的生化性质的参与碳酸氢盐在哺乳动物精子获能。(C)2004 Elsevier B.V.保留所有权利。
This paper deals with the effects of bicarbonate induced signaling pathways on plasma membrane lipid organization and downstream protein signaling, and their role in sperm-egg interactions. It also provides an overview of results that indicate that bicarbonate responses are not related to cell death or apoptosis. The information presented shows that only those sperm cells that have functionally completed maturation in the epididymis are sensitive to bicarbonate whereas immature sperm fail to respond to this physiological challenge. Therefore, it is important to selectively analyze the responsive sperm subpopulations when studying sperm capacitation. Moreover, bicarbonate induced signaling responses differ within the diverse sperm structures (e.g. the tail versus the head). Consequently, dissecting sperm structures and signaling areas from each other deserves more attention in sperm capacitation research. The information discussed was obtained from a variety of mammalian species but the basic bicarbonate-mediated sperm responses are similar in most Eutherian species despite some species to species variations (most notably in kinetics rather than the sequence of events). The objective of the paper is to provide a comparative experimental overview of bicarbonate mediated sperm capacitation in the hope that this information will lead to a better understanding of the complex biochemical nature of the involvement of bicarbonate in mammalian sperm capacitation. (C) 2004 Elsevier B.V. All rights reserved.