Oxidative stress and antioxidant defense in patients with chronic hepatitis C patients before and after pegylated interferon alfa-2b plus ribavirin therapy
Oxidative stress and antioxidant defense in patients with chronic hepatitis C patients before and after pegylated interferon alfa-2b plus ribavirin therapy
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DOI：
10.1186/1479-5876-4-25
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发表时间：
2006-06-20
影响因子：
7.4
通讯作者：
Ahmet, Sayal
中科院分区：
文献类型：
--
作者：
Levent, Goerenek;Ali, Acar;Ahmet, Sayal
背景：氧化应激在丙型肝炎病毒（HCV）感染的发病机制中可能发挥作用。本研究的目的是探讨慢性丙型肝炎（CHC）患者的氧化/抗氧化状态，以及聚乙二醇干扰素α-2b（IFN α-2b）联合利巴韦林（ribavirin）治疗对氧化应激的影响。检测正常对照组和患者组血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）水平、红细胞丙二醛（MDA）水平、红细胞铜锌超氧化物歧化酶（SOD）、红细胞谷胱甘肽过氧化物酶（GSH-Px）活性。结果：CHC患者（n：19）治疗前血清MDA水平显著高于健康对照组（n：28），分别为9.28 ± 1.61，4.20 ± 1.47nmol/ml，P < 0.001。治疗后红细胞MDA含量明显降低（P < 0.001），ALT、AST活性明显降低（P < 0.001）。治疗前CHC患者红细胞中平均抗氧化酶（超氧化物歧化酶和谷胱甘肽过氧化物酶）显著低于对照组（均为p < 0.001）。慢性丙型肝炎患者经聚乙二醇干扰素α-2b和利巴韦林治疗后，SOD、GSH-Px值均显著高于治疗前水平（均P < 0.001）。结论：我们的研究结果表明，慢性丙型肝炎患者处于氧化应激的影响下，与抗氧化酶水平降低有关。在CHC患者的聚乙二醇干扰素α-2b加利巴韦林联合治疗后，这些损害恢复到健康对照的水平。虽然干扰素和利巴韦林不是抗氧化剂，但它们的抗病毒能力可能会减少病毒载量和炎症，并可能通过这种机制减少病毒诱导的氧化应激。
Background: Oxidative stress could play a role in pathogenesis of hepatitis C virus (HCV) infection. The aim of our study is to determine oxidant/antioxidant status of patients with chronic hepatitis C (CHC), and the effect of pegylated interferon alfa-2b plus ribavirin combination therapy on oxidative stress.Methods: Nineteen patients with chronic HCV infection and 28 healthy controls were included in the study. In control and patient groups, serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, erythrocyte malondialdehyde (MDA) levels, erythrocyte CuZn-superoxide dismutase ( SOD), erythrocyte glutathione peroxidase (GSH-Px) activities were measured. After pegylated interferon alfa-2b and ribavirin combination therapy for 48 weeks, these parameters were measured again in the patient group.Results: Serum MDA levels increased significantly in CHC patients (n: 19), before the treatment when compared with healthy subjects ( n: 28) 9.28 +/- 1.61, 4.20 +/- 1.47 nmol/ml, p < 0.001 respectively. MDA concentration decreased significantly ( p < 0.001) after the treatment as well as ALT, AST activity, in erythrocytes of these patients. Average antioxidant enzymes ( superoxide dismutase and glutathione peroxidase) were significantly lower in erythrocytes of patients with CHC before treatment compared with the control group ( both, p < 0.001). Chronic Hepatitis C patients after pegylated interferon alfa-2b and ribavirin therapy showed values of SOD, GSH-Px were significantly higher than pretreatment levels ( both, p < 0.001).Conclusion: Our results show that patients with chronic HCV infection are under the influence of oxidative stress associated with lower levels of antioxidant enzymes. These impairments return to level of healthy controls after pegylated interferon alfa-2b plus ribavirin combination therapy of CHC patients. Although interferon and ribavirin are not antioxidants, their antiviral capacity might reduce viral load, and inflammation, and perhaps through this mechanism might reduce virus-induced oxidative stress.