Human Cytotoxic T Lymphocytes Directed to Seasonal Influenza A Viruses Cross-React with the Newly Emerging H7N9 Virus
Human Cytotoxic T Lymphocytes Directed to Seasonal Influenza A Viruses Cross-React with the Newly Emerging H7N9 Virus
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DOI：
10.1128/jvi.02843-13
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发表时间：
2014-02-01
影响因子：
5.4
通讯作者：
Rimmelzwaan, Guus F.
中科院分区：
文献类型：
--
作者：
van de Sandt, Carolien E.;Kreijtz, Joost H. C. M.;Rimmelzwaan, Guus F.
2013年2月，中国报告了一种新型H7N9亚型甲型流感病毒的人畜共患传播。虽然目前尚未报告持续的人际传播，但担心H7N9病毒会大流行。由于在人群中几乎不存在针对病毒血凝素（HA）球形头部结构域的中和抗体，因此有兴趣确定其他相关的保护作用，例如在季节性甲型流感病毒感染期间引发的交叉反应性CD8(+) T细胞（细胞毒性T淋巴细胞[ctl]）。已知这些病毒特异性CD8(+) T细胞主要识别甲型流感病毒的保守内部蛋白，但尚不清楚它们与新出现的H7N9病毒的交叉反应程度。在这里，我们评估了从hla型研究对象获得的季节性H3N2和H1N1以及大流行性H1N1流感病毒特异性多克隆CD8(+) T细胞与新型H7N9病毒的交叉反应性。CD8(+) T细胞对已知甲型流感病毒表位H7N9变异和H7N9病毒感染细胞的交叉反应性是通过它们的γ干扰素（ifn - γ）应答和裂解活性来测定的。结论表明，除了识别单个H7N9变异表位外，CD8(+) T细胞对季节性流感病毒表现出相当大的交叉反应性。这些交叉反应性CD8(+) T细胞的存在可能对新病毒的感染提供一定的保护。
In February 2013, zoonotic transmission of a novel influenza A virus of the H7N9 subtype was reported in China. Although at present no sustained human-to-human transmission has been reported, a pandemic outbreak of this H7N9 virus is feared. Since neutralizing antibodies to the hemagglutinin (HA) globular head domain of the virus are virtually absent in the human population, there is interest in identifying other correlates of protection, such as cross-reactive CD8(+) T cells (cytotoxic T lymphocytes [CTLs]) elicited during seasonal influenza A virus infections. These virus-specific CD8(+) T cells are known to recognize conserved internal proteins of influenza A viruses predominantly, but it is unknown to what extent they cross-react with the newly emerging H7N9 virus. Here, we assessed the cross-reactivity of seasonal H3N2 and H1N1 and pandemic H1N1 influenza A virus-specific polyclonal CD8(+) T cells, obtained from HLA-typed study subjects, with the novel H7N9 virus. The cross-reactivity of CD8(+) T cells to H7N9 variants of known influenza A virus epitopes and H7N9 virus-infected cells was determined by their gamma interferon (IFN-gamma) response and lytic activity. It was concluded that, apart from recognition of individual H7N9 variant epitopes, CD8(+) T cells to seasonal influenza viruses display considerable cross-reactivity with the novel H7N9 virus. The presence of these cross-reactive CD8(+) T cells may afford some protection against infection with the new virus.