Effect of EpCAM, CD44, CD133 and CD166 expression on patient survival in tumours of the ampulla of Vater
Effect of EpCAM, CD44, CD133 and CD166 expression on patient survival in tumours of the ampulla of Vater
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DOI：
10.1136/jclinpath-2011-200043
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发表时间：
2012-02-01
影响因子：
3.4
通讯作者：
Terracciano, Luigi M.
中科院分区：
文献类型：
--
作者：
Piscuoglio, Salvatore;Lehmann, Frank S.;Terracciano, Luigi M.
研究背景：卵巢系统的癌很少见，可能是由先前存在的腺瘤引起的。根据癌症干细胞（CSC）假说，只有一小部分肿瘤细胞具有启动和发展肿瘤生长的能力。在结直肠癌中，CD 44、CD 133、CD 166和EpCAM被认为是CSC标志蛋白，它们的表达与患者的生存率相关。111腺瘤和152正常粘膜标本安排在组织芯片格式。材料和方法膜免疫反应性为每个蛋白质标记物进行了半定量评估阳性肿瘤细胞的数量超过肿瘤细胞的总数。中位蛋白表达水平用作定义蛋白标志物阳性的截止分数。通过回顾性分析病历、肿瘤登记或直接接触获得包括生存时间在内的临床数据。在所有的标志物中，我们发现从正常组织到肿瘤组织的表达更恒定的趋势。EpCAM表达与更好的患者生存率显著相关。从正常粘膜到腺瘤和癌，CD 44 s、CD 166和CD 133的表达增加与肿瘤进展有关。然而，没有统计学意义上的相关性与survival.Conclusion，我们的研究结果表明，在壶腹癌，EpCAM的表达损失可能与一个更积极的肿瘤表型。
Background Carcinomas of the Vaterian system are rare and presumably arise from pre-existing adenomas. According to the cancer stem cell (CSC) hypothesis, only a small subset of tumor cells has the ability to initiate and develop tumor growth. In colorectal cancer, CD44, CD133, CD166 and EpCAM have been proposed to represent CSC marker proteins and their expression has been shown to correlate with patient survival.Aims To evaluate a potential role of these CSC proteins in tumors of the ampulla of Vater, we investigated their expression in 175 carcinoma, 111 adenoma and 152 normal mucosa specimens arranged in a Tissue Microarray format.Materials and methods Membranous immunoreactivity for each protein marker was scored semi-quantitatively by evaluating the number of positive tumor cells over the total number of tumor cells. Median protein expression levels were used as cut-off scores to define protein marker positivity. Clinical data including survival time were obtained by retrospective analysis of medical records, tumor registries or direct contact.Results The expression of all evaluated marker proteins differed significantly between normal mucosa, adenoma and carcinoma samples. In all markers, we found a tendency towards more constant expression from normal to neoplastic tissue. EpCAM expression was significantly correlated with better patient survival. The increased expression of CD44s, CD166 and CD133 from normal mucosa samples to adenoma and carcinoma was linked to tumor progression. However, there was no statistically significant correlation with survival.Conclusion Our findings indicate, that in ampullary carcinomas, loss of expression of EpCAM may be linked to a more aggressive tumor phenotype.