Immunosenescence and inflammation characterize chronic heart failure patients with more advanced disease
Immunosenescence and inflammation characterize chronic heart failure patients with more advanced disease
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DOI：
10.1016/j.ijcard.2014.04.128
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发表时间：
2014-07-01
影响因子：
3.5
通讯作者：
Alonso-Arias, Rebeca
中科院分区：
文献类型：
--
作者：
Antonio Moro-Garcia, Marco;Echeverria, Ainara;Alonso-Arias, Rebeca
背景：慢性心力衰竭 (CHF) 的特点是炎症状态，细胞因子（如 IL-6）水平较高。我们假设 CHF 患者可能因炎症而出现免疫衰老，这可能与疾病的更严重阶段有关。 方法和结果：我们比较了 58 名老年 CHF 患者 (ECHF)、40 名年轻 CHF 患者 (YCHF)、60 名健康老年对照 (HEC) 和 40 名健康年轻对照 (HYC) 的免疫学特征。我们通过流式细胞术表征白细胞和淋巴细胞亚群，并通过 ELISA 表征 IL-6 浓度。 CHF 的程度根据功能和/或形态学标准进行分类：纽约心脏协会功能分级、AHA/ACC 心力衰竭分期、左心室射血分数和左心室肥厚。 CHF患者白细胞、中性粒细胞和单核细胞数量增加，但淋巴细胞数量减少。 CHF 患者的 B 细胞和 CD4+ T 细胞水平显着降低，YCHF 患者的 NK 细胞增加，仅 ECHF 患者的 CD8+ T 细胞增加。 CHF 与 CD4+ 和 CD8+ T 淋巴细胞亚群的高度分化相关。 T 淋巴细胞亚群的衰老和高 IL-6 水平与较差的临床状态相关。 IL-6 还与高度分化的 T 淋巴细胞数量及其加速衰老呈正相关。结论：我们得出结论，CHF 患者比年龄匹配的健康对照者表现出更高程度的免疫衰老。临床状态晚期的患者 T 淋巴细胞分化和 IL-6 水平增加，由于免疫系统加速老化，适应性免疫反应受损，可能导致疾病受损。 (C) 2014 Elsevier Ireland Ltd. 保留所有权利。
Background: Chronic heart failure (CHF) is characterized by an inflammatory status with high levels of cytokines such as IL-6. We hypothesized that patients with CHF may develop immunosenescence due to inflammation and that this may be associated with a worse stage of the disease.Methods and results: We compared the immunological features of 58 elderly CHF patients (ECHF), 40 young CHF patients (YCHF), 60 healthy elderly controls (HEC) and 40 healthy young controls (HYC). We characterized leukocyte and lymphocyte subpopulations by flow cytometry, and IL-6 concentration by ELISA. The extent of CHF was classified according to functional and/or morphological criteria: New York Heart Association functional class, AHA/ACC heart failure stages, left ventricular ejection fraction, and left ventricular hypertrophy. CHF patients showed an increased number of leukocytes, neutrophils and monocytes, but a decreased number of lymphocytes. CHF patients had significantly lower levels of B-cells and CD4+ T-cells, increased NK-cells in YCHF, and increased CD8+ T-cells only in ECHF. CHF was associated with high differentiation in CD4+ and CD8+ T-lymphocyte subsets. Aging of T-lymphocyte subpopulations and high IL-6 levels were associated with a worse clinical status. IL-6 also correlated positively with the number of highly differentiated T-lymphocytes and with their accelerated aging.Conclusions: We conclude that CHF patients show a higher degree of immunosenescence than age-matched healthy controls. T-lymphocyte differentiation and IL-6 levels are increased in patients with an advanced clinical status and may contribute to disease impairment through a compromised adaptive immune response due to accelerated aging of their immune system. (C) 2014 Elsevier Ireland Ltd. All rights reserved.