Effects of prolactin and glycosylated prolactin on (pro)insulin synthesis and insulin release from cultured rat pancreatic islets.
Effects of prolactin and glycosylated prolactin on (pro)insulin synthesis and insulin release from cultured rat pancreatic islets.
复制标题
催乳素和糖基化催乳素对培养的大鼠胰岛的胰岛素（原）合成和胰岛素释放的影响。
DOI：
10.1097/00006676-199001000-00015
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发表时间：
1990
期刊：
影响因子：
2.9
通讯作者：
Lewis,UJ
中科院分区：
文献类型：
--
作者：
Markoff,E;Beattie,GM;Hayek,A;Lewis,UJ
为了研究催乳素的非糖基化和糖基化形式对胰岛中胰岛素含量和分泌的可能差异效应，将新生大鼠胰岛在体外暴露于2 [mu] g/ml的非糖基化绵羊催乳素（oPRL）或2 [mu] l的糖基化oPRL（G-oPRL）6天。在6天的培养期内，oPRL刺激释放到培养基中的胰岛素总量显著增加（p< 0.01）;然而，G-oPRL没有影响。在oPRL存在下培养6天的胰岛显示每个胰岛的DNA量没有增加。然而，暴露于oPRL的胰岛合成的总蛋白量显著增加（p< 0.007）。这些结果表明，oPRL对新生大鼠胰岛细胞的作用是一种非特异性的作用。PRL的非糖基化形式可能通过促进蛋白质合成而在B细胞功能中发挥作用，这导致胰岛素合成增加。
In order to study the possible differential effects of the nonglycosylated and glycosylated forms of prolactin on insulin content and secretion in pancreatic islets, neonatal rat pancreatic islets were exposed for 6 days in vitro to 2 [mu] g/ml of nonglycosylated ovine prolactin (oPRL), or to 2 [mu] l of glycosylated oPRL (G-oPRL). oPRL stimulated a significant increase (p< 0.01) in the total amount of insulin released into the medium over the 6 day culture period; however, G-oPRL had no effect. Islets cultured for 6 days in the presence of oPRL showed no increase in the amount of DNA per islet. However, there was a significant (p< 0.007) increase in the amount of total protein synthesized by the islets exposed to oPRL. These findings suggest that the effect of oPRL on neonatal rat pancreatic islet cells is a nonspecific effect. The nonglycosylated form of PRL may play a role in B-cell function by promoting protein synthesis, which results in augmented insulin synthesis.