α-Tubulin and F-actin distribution during microsporogenesis in a 2n pollen producer of Solanum
α-Tubulin and F-actin distribution during microsporogenesis in a 2n pollen producer of Solanum
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DOI：
10.1139/g98-063
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发表时间：
1998-10
期刊：
影响因子：
3.1
通讯作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
中科院分区：
文献类型：
--
作者：
G. Genualdo;A. Errico;A. Tiezzi;C. Conicella
采用α-微管蛋白免疫定位和F-肌动蛋白罗丹明-鬼笔环肽染色技术，研究了茄属2n花粉产生菌小孢子发生过程中微管和微丝形态的时空变化，探讨了减数分裂纺锤体和胞质分裂的异常及其与2n花粉形成的关系。在茄属植物中，MT和MF通常表现出与其他双子叶植物中描述的同时胞质分裂相似的定位模式。然而，在减数分裂II和胞质分裂中观察到与2n花粉相关的MT和MF的空间构型的偏差。在减数分裂II中，MT和MF以平行方向定位于纺锤体中。相反，在大多数正常的性母细胞中，两个纺锤体被认为是相互垂直的。平行纺锤体改变了减数分裂后细胞核的位置，使其由四面体排列变为单平面排列。随后，规则的放射状MT系统的形成受到抑制，只有两个MT阵列，它们平行地放置在一个平面上，在减数分裂结束时形成的核之间。在两个MT阵列上形成单细胞板，产生含有2n个小孢子的二分体。
Spatial and temporal changes in configurations of microtubules (MTs) and microfilaments (MFs) were determined during microsporogenesis, using α-tubulin immunolocalization and rhodamine-phalloidin staining of F- actin, in a 2n pollen producer of Solanum, to assess the anomalies in meiotic spindles and cytokinesis and their relationships to 2n pollen formation. In Solanum, MTs and MFs generally showed patterns of localization similar to those described in other dicotyledons with simultaneous cytokinesis. However, deviations in spatial configurations of both MTs and MFs that are related to 2n pollen were observed in meiosis II and in cytokinesis. MTs and MFs localized in spindles in parallel orientation at meiosis II. In contrast, in the majority of normal meiocytes, the two spindles were seen to be perpendicular to each other. The parallel spindles altered the position of the postmeiotic nuclei, causing a uniplanar instead of a tetrahedral arrangement. Subsequently, the formation of regular radial MT systems was suppressed and only two MT arrays, which lay parallel on one plane, formed between the nuclei at the end of meiosis. A single cell plate formed across the two MT arrays, giving rise to a dyad containing 2n microspores.