Effects of printing conditions on cell distribution within microspheres during inkjet-based bioprinting
Effects of printing conditions on cell distribution within microspheres during inkjet-based bioprinting
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DOI：
10.1063/1.5116371
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发表时间：
2019-09
期刊：
影响因子：
1.6
通讯作者：
Heqi Xu;Jazzmin Casillas;Changxue Xu
中科院分区：
文献类型：
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作者：
Heqi Xu;Jazzmin Casillas;Changxue Xu
基于喷墨的生物打印已被广泛用于组织工程和药物筛选和递送中的各种应用。用于喷墨生物打印的典型生物墨水由生物材料和活细胞组成。在喷墨生物打印过程中，载有细胞的生物墨水从喷墨分配器中喷出，以形成包封有细胞的微球。微球内的细胞分布被定义为微球内细胞数量的分布。本文重点研究了喷墨打印细胞墨水过程中聚合物浓度、激发电压和细胞浓度对微球内细胞分布的影响。正态分布已被用来拟合实验结果，以获得分布的平均值和标准差。结果表明，微球内细胞分布随细胞浓度、海藻酸钠浓度和激发电压的增加而增加。
Inkjet-based bioprinting have been widely employed in a variety of applications in tissue engineering and drug screening and delivery. The typical bioink used in inkjet bioprinting consists of biological materials and living cells. During inkjet bioprinting, the cell-laden bioink is ejected out from the inkjet dispenser to form microspheres with cells encapsulated. The cell distribution within microspheres is defined as the distribution of cell number within the microspheres. The paper focuses on the effects of polymer concentration, excitation voltage, and cell concentration on the cell distribution within microspheres during inkjet printing of cell-laden bioink. The normal distribution has been utilized to fit the experimental results to obtain the mean and standard deviation of the distribution. It is found that the cell distribution within the microspheres increases with the increase of the cell concentration, sodium alginate concentration, and the excitation voltage.