Vertical Flow Assay for Inflammatory Biomarkers Based on Nanofluidic Channel Array and SERS Nanotags
Vertical Flow Assay for Inflammatory Biomarkers Based on Nanofluidic Channel Array and SERS Nanotags
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DOI：
10.1002/smll.202002801
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发表时间：
2020-06-22
期刊：
影响因子：
13.3
通讯作者：
Zhao, Xiangwei
中科院分区：
文献类型：
--
作者：
Chen, Ruipeng;Du, Xin;Zhao, Xiangwei
因此，对高通量、高灵敏度的炎症感染诊断检测方法的开发具有很大的需求。本文中，提出了一种垂直流测定系统，其具有功能化纳米多孔阳极氧化铝（AAO）作为传感膜，以及编码的核-壳表面增强拉曼散射（Sers）纳米标签作为用于多种炎症生物标志物检测的标记物。开发多孔AAO上的2 × 2测试阵列，并用多种捕获抗体修饰，以从样品中捕获炎性生物标志物。由于AAO膜的高表面积与体积比及其对等离子体激元耦合的影响，增强了编码的核-壳Sers纳米标签的电磁场。C反应蛋白、白细胞介素-6、血清淀粉样蛋白A和降钙素原的检测限分别为53.4、4.72、48.3和7.53 fg mL（-1），线性动态范围至少跨越5个数量级。此外，所提出的方法也显示出可接受的准确度和重复性的检测临床样品。因此，这种方法有望成为设施有限地区疾病诊断的一种强大的护理点检测工具。
There is a great demand for the development of detection assays for inflammation infection diagnosis with high throughput and ultrasensitivity. Herein, a vertical flow assay system with functionalized nanoporous anodic aluminum oxide (AAO) as sensing membrane, and encoded core-shell surface enhanced Raman scattering (SERS) nanotags as labels for multiple inflammatory biomarkers detection is presented. A 2 x 2 test array on the porous AAO is developed and modified with multiple capture antibodies to capture inflammatory biomarkers from samples. Due to the high surface area to volume ratio of the AAO membrane, and its influence on plasmonic coupling, the electromagnetic field of the encoded core-shell SERS nanotags is enhanced. Detection limits of 53.4, 4.72, 48.3, and 7.53 fg mL(-1)are realized for C reactive protein, interleukin-6, serum amyloid A, and procalcitonin, respectively, with a linear dynamic range spanning at least five orders of magnitude. In addition, the proposed method also shows acceptable accuracy and repeatability for the detection of clinical samples. Therefore, this approach is expected to be a powerful point of care testing tool for disease diagnosis in facility limited areas.