Accessible hyaluronan receptors identical to ICAM-1 in mouse mast-cell tumours
Accessible hyaluronan receptors identical to ICAM-1 in mouse mast-cell tumours
复制标题
小鼠肥大细胞肿瘤中可接近的透明质酸受体与 ICAM-1 相同
作者：
S. Gustafson;Tomas Björkman;N. Forsberg;T. Lind;T. Wikström;K. Lidholt
透明质酸（HA）受体（R）的免疫组织化学研究，最初发现在肝内皮细胞（LEC）和相关的细胞间粘附分子1（ICAM-1），显示针对HARLEC（HA受体LEC）的多克隆抗体也染色小鼠肥大细胞瘤的结构，主要是血管。为了测试静脉注射HA是否可以靶向体内肿瘤受体，在一条后腿肌肉中携带接种肥大细胞瘤的小鼠尾静脉注射125 I-酪氨酸（T）标记的HA，并在注射后75分钟处死，检查器官和组织的放射性。当注射超过肝脏结合能力的剂量时，发现肿瘤组织内的放射性显著增加（高达5倍）。对照组和肿瘤组织之间的重量调整差异对于较小的肿瘤更大，可能是由于较大肿瘤的坏死。HA染色的肿瘤动物接受125 I-T-HA，显示HA的区域也染色较弱的ICAM-1使用单克隆抗体。透明质酸酶处理切片后，ICAM-1染色显著增加，表明HA与这些受体结合，从而阻断抗体识别。
Immunohistochemical studies of the hyaluronan (HA)-receptor (R), originally found on liver endothelial cells (LEC) and related to the intercellular adhesion molecule 1 (ICAM-1), showed that polyclonal antibodies against HARLEC (HA receptor on LEC) also stain structures in mouse mastocytomas, mainly vessels. To test if intravenously administered HA might target the tumour receptorsin vivo, mice carrying an inoculated mastocytoma in one hind leg muscle were injected in the tail vein with125I-tyrosine (T)-labelled HA and killed 75 min after injection when organs and tissues were checked for radioactivity. When doses exceeding the binding capacity of the liver were injected, a significant increase in radioactivity (up to five-fold) within the tumour tissue was found. The weight adjusted difference between control and tumour tissue was greater for smaller tumours, probably due to necrosis in the larger. HA-staining of tumours from animals receiving125I-T-HA, showed HA in areas that also stained weakly for ICAM-1 using monoclonal antibodies. ICAM-1 staining was dramatically increased after hyaluronidase treatment of the sections, indicating that the HA is bound to these receptors and thereby blocks antibody recognition.
角膜内皮透明质酸酶：在前房透明质酸分解代谢中发挥作用。
影响因子：
2.8
作者：
Schwartz,DM;Jumper,MD;Lui,GM;Dang,S;Schuster,S;Stern,R
通讯作者：
Stern,R
影响因子：
4.1
作者：
MCGARY, CT;RAJA, RH;WEIGEL, PH
通讯作者：
WEIGEL, PH