Introduction of new subtypes and variants of hepatitis C virus genotype 4 in South Africa
Introduction of new subtypes and variants of hepatitis C virus genotype 4 in South Africa
复制标题
DOI：
10.1002/jmv.23215
复制
发表时间：
2012-04-01
影响因子：
12.7
通讯作者：
Mphahlele, M. Jeffery
中科院分区：
文献类型：
--
作者：
Gededzha, Maemu P.;Selabe, Selokela G.;Mphahlele, M. Jeffery
丙型肝炎病毒（HCV）基因型是疾病进展和治疗反应的重要预测因子。这项描述性研究调查了南非HCV的序列多样性和基因型，基于对52例患者的60个序列的5'非翻译区（UTR）、C/E1和NS 5 B区的比较分析。研究人群的基因型分布如下：54%（28/52）为基因型5，19%（10/52）为基因型1，19%（10/52）为基因型4，2%（1/52）为基因型3。52个个体中的3个（6%）感染了基于5 'UTR的多种基因型。5 'UTR的系统发育分析在确定基因型方面是准确的，而C/E1和NS 5 B编码区能够区分基因型和亚型，包括离群组。此外，本研究观察到HCV的不同变体的存在，这些变体与确认的基因型4亚型不同。在南非，这项分析首次显示了HCV亚型4k、4 q和4 r的存在，以及NS 5 B内基因型内重组4l/4 q的证据。总之，虽然基因型5a仍然是南非的主要基因型，但当前的研究表明南非引入了新的亚型并存在基因型4的变体。医学病毒学杂志84：601607，2012。(C)2011 Wiley Periodicals，Inc.
Hepatitis C virus (HCV) genotype is an important predictor of disease progression and treatment response. This descriptive study investigated the sequence diversity and genotypes of HCV in South Africa based on comparative analysis of the 5' untranslated region (UTR), C/E1, and NS5B regions of 60 sequences from 52 patients. Genotype distribution in the studied population was as follows: 54% (28/52) were genotype 5, 19% (10/52) were genotype 1, 19% (10/52) were genotype 4, and 2% (1/52) were genotype 3. Three of 52 (6%) individuals were infected with multiple genotypes based on the 5'UTR. Phylogenetic analysis of the 5'UTR was accurate in determining genotypes, while the C/E1 and NS5B coding region was able to differentiate both genotypes and subtypes, including an outlier group. Furthermore, this study observed the existence of distinct variants of HCV which were divergent from confirmed genotype 4 subtypes. For the first time in South Africa, this analysis has shown the presence of HCV subtypes 4k, 4q, and 4r, as well as evidence of intragenotypic recombinant 4l/4q within NS5B. In conclusion, while genotype 5a remains the predominant genotype in South Africa, the current study indicates the introduction of new subtypes and existence of variants of genotype 4 in South Africa. J. Med. Virol. 84:601607, 2012. (C) 2011 Wiley Periodicals, Inc.