Sequential formation of regioselective disulfide bonds in synthetic peptides with multiple disulfide bonds.
Sequential formation of regioselective disulfide bonds in synthetic peptides with multiple disulfide bonds.
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DOI：
10.1007/978-1-62703-544-6_5
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发表时间：
2015-05
影响因子：
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通讯作者：
F. Shabanpoor;M. Hossain;F. Lin;J. Wade
中科院分区：
文献类型：
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作者：
F. Shabanpoor;M. Hossain;F. Lin;J. Wade
已经开发了许多用于在重组DNA衍生的和化学合成的肽和蛋白质中形成二硫键的方法，但只有少数方法被广泛接受。用于在合成肽和蛋白质中形成二硫化物的方法的选择需要以这样的方式针对每种单独的多肽进行定制，使得反应条件是选择性的、有效的和安全的，并且给出最大的产率。在这里，我们描述了顺序形成的三个二硫键区域选择性已被优化的合成的两链，异源二聚体的多肽成员的胰岛素松弛素超家族。
Numerous methods have been developed for the formation of disulfide bonds in recombinant DNA-derived and chemically synthesized peptides and proteins, but only a few have found widespread acceptance. The choice of method(s) for formation of disulfide in synthetic peptides and proteins needs to be tailored for each individual polypeptide in such a way so that the reaction conditions are selective, efficient, and safe and give the maximum yield. Here we describe the sequential formation of three disulfide bonds regioselectively which has been optimized for the synthesis of two-chained, heterodimeric polypeptide members of the insulin–relaxin superfamily.