Low intensity ultrasound-induced apoptosis in human gastric carcinoma cells
Low intensity ultrasound-induced apoptosis in human gastric carcinoma cells
复制标题
低强度超声诱导人胃癌细胞凋亡
DOI：
10.3748/wjg.14.4873
复制
发表时间：
2008-08-21
影响因子：
4.3
通讯作者：
Zheng, Zhao-Bin
中科院分区：
文献类型：
--
作者：
Feng, Yi;Tian, Zhong-Min;Zheng, Zhao-Bin
目的：为探讨低强度超声（low intensity ultrasound，US）诱导人胃癌细胞凋亡及其可能机制，为胃癌的治疗提供新的途径.方法：体外培养人胃癌细胞SGC-7901，用不同强度的低强度US照射10 min，照射后不同孵育时间.台盼蓝染色观察细胞形态学变化，异硫氰酸荧光素（FITC）-Annexin V/PI双染流式细胞仪（FCM）检测早期凋亡细胞百分率。采用双向电泳（2DE）技术分析蛋白质图谱，并采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）技术鉴定超声辐照后细胞中差异表达的蛋白质。结果：经12.0W/cm（2）超声照射后，培养12 h，凋亡细胞比例增加约10%。随着超声强度的增加，细胞早期凋亡和继发性坏死的比例增加。随着照射后培养时间的延长，凋亡细胞逐渐增多，在12 h左右达到高峰。在超声辐照后出现了几种新的蛋白质，并上调或下调了2倍以上。结论：低强度超声能诱导人胃癌细胞凋亡，且与热休克蛋白（HSPs）有关。超声诱导的细胞凋亡与超声强度/培养时间有关。超声波诱导的细胞凋亡可能是半胱天冬酶依赖性的，内质网应激也可能参与其中。蛋白质组学实验系统有助于发现超声波照射后肿瘤细胞蛋白质的变化，为肿瘤治疗提供新的思路。(c)2008年，WJG出版社。All rights reserved.
AIM: To investigate the low intensity ultrasound (US)induced apoptosis in human gastric carcinoma cells and its potential mechanism and to suggest a new therapeutic approach to gastric carcinoma.METHODS: Human SGC-7901 gastric carcinoma cells were cultured in vitro and irradiated by low intensity US for 10 min at different intensities with different incubation times after irradiation. Morphologic changes were examined under microscope with trypan blue staining and then the percentage of early apoptotic cells was detected by flow cytometry (FCM) with double staining of fluorescein isothiocyanate (FITC)-Annexin V/propidium iodide (PI). Two-dimensional electrophoresis (2DE) was used to get the protein profile and some proteins differently expressed after US irradiation were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). Functional analysis was performed to investigate the mechanism of US-induced cell apoptosis.RESULTS: The percentage of apoptotic cells increased about 10% after US irradiation (12.0 W/cm(2), 12 h culture). The percentage of early apoptosis and secondary necrosis in the US-irradiated cells increased with the increased US intensity. Moreover, apoptotic cells increased with the increased culture time after US irradiation and reached its maximum at about 12 h. Several new proteins appeared after US irradiation and were up or down regulated more than 2 times. Some heat shock proteins (HSPs) were found to be associated with the signal process simulating the apoptosis of cells.CONCLUSION: Low intensity US could induce apoptosis in human gastric carcinoma cells. US-induced apoptosis is related to US intensity/culture time. US-induced apoptosis may be caspases-dependent and endoplasmic reticulum (ER) stress-triggered apoptosis may also contribute to it. Proteomic experimental system is useful in finding the protein alteration in carcinoma cells after US irradiation, helping to develop a new cancer therapy. (c) 2008 The WJG Press. All rights reserved.