Physical map of the Salmonella typhimurium histidine transport operon: correlation with the genetic map.
Physical map of the Salmonella typhimurium histidine transport operon: correlation with the genetic map.
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鼠伤寒沙门氏菌组氨酸转运操纵子的物理图谱：与遗传图谱的相关性。
DOI：
10.1128/jb.147.2.401-409.1981
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发表时间：
1981
影响因子：
3.2
通讯作者：
Ames,GF
中科院分区：
文献类型：
--
作者：
Ardeshir,F;Higgins,CF;Ames,GF
展示了鼠伤寒沙门氏菌脱氧核糖核酸 (DNA) 的 12.4 KB EcoRI 片段的详细限制性图谱，其中包含整个组氨酸转运操纵子和 argT 基因。在质粒载体中构建了鼠伤寒沙门氏菌转运操纵子特定区域的亚克隆。通过将来自携带每个缺失的菌株的染色体DNA的限制性消化物与克隆的转运操纵子DNA作为探针进行杂交，获得了限制性图谱和遗传定义的缺失位置之间的准确关联。这些数据用于将组氨酸转运基因定位在克隆的 12.4 KB DNA 片段上。
A detailed restriction map of a 12.4-kilobase EcoRI fragment of Salmonella typhimurium deoxyribonucleic acid (DNA) containing the entire histidine transport operon and the argT gene is presented. Subclones of specific regions of the transport operon of S. typhimurium were constructed in plasmid vectors. An accurate correlation between the restriction map and the location of genetically defined deletions was obtained by hybridizing restriction digests of chromosomal DNA from strains carrying each deletion with cloned transport operon DNA as a probe. These data were used to position the histidine transport genes on the cloned 12.4-kilobase fragment of DNA.