Localization of Id2 mRNA in the adult mouse brain
Localization of Id2 mRNA in the adult mouse brain
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DOI：
10.1016/j.brainres.2005.12.048
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发表时间：
2006-02-16
期刊：
影响因子：
2.9
通讯作者：
Yokota, Y
中科院分区：
文献类型：
--
作者：
Kitajima, K;Takahashi, R;Yokota, Y
Id蛋白是碱性螺旋-环-螺旋转录因子的负调控因子，参与细胞分化和增殖。Id基因家族的四个成员不仅在胚胎而且在成年的各种哺乳动物器官中表现出密切相关但不同的表达模式。其中，Id 2已知在成年小鼠大脑皮质层的浦肯野细胞和神经元中表达，表明Id 2参与成年小鼠的一些神经功能。为了深入了解Id 2在神经系统中的作用，我们研究了Id 2 mRNA表达细胞在成年小鼠脑中的定位详细的放射性标记的反义探针原位杂交，并与其他Id基因家族成员的定位进行了比较。结果表明，Id 2 mRNA在更多不同的脑区比以前报道的。这些区域包括杏仁复合体、尾壳核、苍白球、黑质网状部、视交叉上核和室下区前部。这些结果提示Id 2可能在神经活动和认知功能中起作用。另一方面，Id 1几乎检测不到。Id 3的表达呈弥漫性分布，但在齿状回分子层和外囊等特定区域有高表达。Id 4 mRNA在尾壳核和杏仁外侧核等区域有表达。因此，Id 2的表达模式不同于其他Id基因家族成员的表达模式。(c)2005 Elsevier B. V.保留所有权利。
Id proteins are negative regulators of basic helix-loop-helix transcription factors and are involved in cellular differentiation and proliferation. Four members of the Id gene family exhibit closely related but distinct expression patterns in various mammalian organs of not only embryos but also adults. Among them, Id2 is known to be expressed in Purkinje cells and neurons in the cortical layers of the adult mouse brain, suggesting that Id2 is involved in some neural functions in the adult. To get insight into the role of Id2 in the nervous system, we investigated the localization of Id2 mRNA-expressing cells in the adult mouse brain in detail by in situ hybridization with the radiolabeled antisense probe and compared it with the localization of other Id gene family members. The results indicated that Id2 mRNA is detected in more varied brain regions than previously reported. These regions include the amygdaloid complex, caudate putamen, globus pallidus, substantia nigra pars reticulata, suprachiasmatic nucleus, and the anterior part of the subventricular zone. These results suggest the possibility that Id2 plays a role in the neural activity and cognitive functions. On the other hand, Id1 was barely detectable. Although moderate or low expression of Id3 was observed diffusely, high expression was observed in some specific regions including the molecular layer of the dentate gyrus and the external capsule. Id4 mRNA was detected in the regions such as the caudate putamen and the lateral amygdaloid nucleus. Thus, the expression pattern of Id2 is distinct from those of other Id gene family members. (c) 2005 Elsevier B.V. All rights reserved.