The effect of the length of direct repeats and the presence of palindromes on deletion between directly repeated DNA sequences in bacteriophage T7.
The effect of the length of direct repeats and the presence of palindromes on deletion between directly repeated DNA sequences in bacteriophage T7.
复制标题
噬菌体 T7 中直接重复序列的长度和回文结构的存在对直接重复 DNA 序列之间删除的影响。
DOI：
10.1093/nar/19.14.3901
复制
发表时间：
1991
影响因子：
14.9
通讯作者：
Masker,W
中科院分区：
文献类型：
--
作者：
Pierce,JC;Kong,D;Masker,W
噬菌体 T7 中直接重复 DNA 序列之间的遗传缺失频率被测量为同向重复长度的函数。噬菌体 T7 的非必需连接酶基因（基因 1.3）被不同长度的同向重复序列括起来的合成 DNA 片段打断。当同向重复序列长度小于 6 bp 时，这些 76 bp 长插入片段的缺失太低而无法测量。然而，随着重复长度从 8 bp 增加到 20 bp，具有较长直接重复序列的插入片段的缺失频率呈指数增加。当在插入片段中间设计反向重复序列（回文）时，10 bp 直接重复序列之间的缺失频率基本上没有增加。但是，相同的回文序列将 5 bp 直接重复之间的删除频率增加了至少两个数量级。因此，在该系统中，端点处的同源性是比直接重复之间是否存在回文更重要的删除频率决定因素。
The frequency of genetic deletion between directly repeated DNA sequences in bacteriophage T7 was measured as a function of the length of the direct repeat. The non-essential ligase gene (gene 1.3) of bacteriophage T7 was Interrupted with pieces of synthetic DNA bracketed by direct repeats of various lengths. Deletion of these 76 bp long inserts was too low to be measured when the direct repeats were less than 6 bp long. However, the frequency of deletion of inserts with longer direct repeats increased exponentially as the length of the repeats increased from 8 to 20 bp. When Inverted repeats (palindromes) were designed in the midst of the insert there was essentially no increase In deletion frequency between 10 bp direct repeats. But, the same palindromic sequences increased the deletion frequency between 5 bp direct repeats by at least two orders of magnitude. Thus, in this system homology at the endpoints Is a more important determinant of deletion frequency than is the presence of palindromes between the direct repeats.
DNA复制和突变
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
R. Moses;W. Summers
通讯作者：
W. Summers
DNA 复制和诱变：Moses，R.E. & Summers，W.C. — 1 卷（18 × 26 厘米），493 + xxii 页，华盛顿，1988 年。 奖金：会员：39.00 美元/非会员：59.00 美元
DOI：
--
发表时间：
1988
期刊：
影响因子：
--
作者：
M. Hofnung
通讯作者：
M. Hofnung