UXT is a novel centrosomal protein essential for cell viability
UXT is a novel centrosomal protein essential for cell viability
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DOI：
10.1091/mbc.e05-08-0705
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发表时间：
2005-12-01
影响因子：
3.3
通讯作者：
Greene, MI
中科院分区：
文献类型：
--
作者：
Zhao, HW;Wang, Q;Greene, MI
普遍表达转录本(UXT)是一种折叠前蛋白，已被认为与人类肿瘤的发生有关。在这里，我们发现UXT在一些人类肿瘤组织中过表达，但在匹配的正常组织中不表达。我们证明UXT定位于人类中心体，并与γ-微管蛋白相关。此外，UXT的过度表达破坏了中心体结构。此外，通过小干扰RNA敲除UXT蛋白的表达会导致细胞死亡。综上所述，我们的发现表明UXT是中心体的一个组成部分，对细胞活力是必不可少的。我们认为，UXT可能通过破坏调节的中心体功能来促进转化。
Ubiquitously expressed transcript (UXT) is a prefoldinlike protein that has been suggested to be involved in human tumorigenesis. Here, we have found that UXT is overexpressed in a number of human tumor tissues but not in the matching normal tissues. We demonstrate that UXT is located in human centrosomes and is associated with gamma-tubulin. In addition, overexpression of UXT disrupts centrosome structure. Furthermore, abrogation of UXT protein expression by small interfering RNA knockdown leads to cell death. Together, our findings suggest that UXT is a component of centrosome and is essential for cell viability. We propose that UXT may facilitate transformation by corrupting regulated centrosome functions.