Specific Bacterial Cell Wall Components Influence the Stability of Coxsackievirus B3
Specific Bacterial Cell Wall Components Influence the Stability of Coxsackievirus B3
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DOI：
10.1128/jvi.01424-21
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发表时间：
2021-11-01
影响因子：
5.4
通讯作者：
Robinson, Christopher M.
中科院分区：
文献类型：
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作者：
Dhalech, Adeeba H.;Fuller, Tara D.;Robinson, Christopher M.
肠道病毒感染哺乳动物胃肠道并导致全世界显着的发病率和死亡率。数据表明，肠道病毒可以利用肠道细菌促进病毒复制和发病。然而，肠道病毒和细菌之间的精确相互作用尚不清楚。在这里，我们研究了细菌和柯萨奇病毒 B3 之间的相互作用，柯萨奇病毒 B3 是一种来自小核糖核酸病毒家族的肠道病毒。我们发现细菌在体外增强了柯萨奇病毒 B3 (CVB3) 的感染性。值得注意的是，需要特定的细菌，如革兰氏阴性肠道沙门氏菌，而不是大肠杆菌，可以增强 CVB3 的感染性和稳定性。对肠沙门氏菌和大肠杆菌的细胞壁成分的研究表明，O-抗原或脂多糖核心（革兰氏阴性细菌细胞壁的主要成分）中的结构是肠沙门氏菌增强 CVB3 所必需的。为了确定这些要求对于类似的肠道病毒是否是必要的，我们研究了肠沙门氏菌和大肠杆菌是否增强了脊髓灰质炎病毒（小核糖核酸病毒家族中的另一种肠道病毒）的感染性。我们发现，虽然大肠杆菌没有增强 CVB3 的感染性，但大肠杆菌增强了脊髓灰质炎病毒的感染性。总体而言，这些数据表明，不同的细菌增强了 CVB3 的感染性和稳定性，并且特定的肠道病毒与特定细菌物种的相互作用可能有不同的要求。 重要性 先前的数据表明，几种肠道病毒利用细菌来促进肠道感染和病毒稳定性。在这里，我们证明需要特定的细菌和细菌细胞壁成分来增强柯萨奇病毒 B3 的体外感染性和稳定性。这些要求可能是肠道病毒特有的，因为 CVB3 的细菌不同于脊髓灰质炎病毒（一种密切相关的病毒）。因此，这些数据表明特定细菌及其细胞壁成分决定了与各种肠道病毒以不同机制的相互作用。
Enteric viruses infect the mammalian gastrointestinal tract and lead to significant morbidity and mortality worldwide. Data indicate that enteric viruses can utilize intestinal bacteria to promote viral replication and pathogenesis. However, the precise interactions between enteric viruses and bacteria are unknown. Here, we examined the interaction between bacteria and coxsackievirus B3, an enteric virus from the picornavirus family. We found that bacteria enhance the infectivity of coxsackievirus B3 (CVB3) in vitro. Notably, specific bacteria are required, as Gram-negative Salmonella enterica, but not Escherichia coli, enhanced CVB3 infectivity and stability. Investigating the cell wall components of both S. enterica and E. coli revealed that structures in the O-antigen or core of lipopolysaccharide, a major component of the Gram-negative bacterial cell wall, were required for S. enterica to enhance CVB3. To determine if these requirements were necessary for similar enteric viruses, we investigated if S. enterica and E. coli enhanced infectivity of poliovirus, another enteric virus in the picornavirus family. We found that while E. coli did not enhance the infectivity of CVB3, E. coli enhanced poliovirus infectivity. Overall, these data indicate that distinct bacteria enhance CVB3 infectivity and stability, and specific enteric viruses may have differing requirements for their interactions with specific bacterial species.IMPORTANCE Previous data indicate that several enteric viruses utilize bacteria to promote intestinal infection and viral stability. Here, we show that specific bacteria and bacterial cell wall components are required to enhance infectivity and stability of coxsackievirus B3 in vitro. These requirements are likely enteric virus specific, as the bacteria for CVB3 differ from poliovirus, a closely related virus. Therefore, these data indicate that specific bacteria and their cell wall components dictate the interaction with various enteric viruses in distinct mechanisms.