Identification of source of calcium in HeLa cells by femtosecond laser excitation
Identification of source of calcium in HeLa cells by femtosecond laser excitation
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DOI：
10.1117/1.3485741
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发表时间：
2010-09-01
影响因子：
3.5
通讯作者：
Chan, Kam Tai
中科院分区：
文献类型：
--
作者：
He, Hao;Kong, Siu Kai;Chan, Kam Tai
钙是细胞内重要的信使，其在亚细胞水平的储存和扩散动力学尚不清楚。通过飞秒激光照射不同介质中的HeLa细胞，诱导受控的缓慢亚细胞Ca 2+释放，细胞质被确定为主要的细胞内Ca 2+储存，细胞核是次要的储存，细胞外Ca 2+也有助于细胞总Ca 2+水平。此外，细胞质或细胞核中释放的Ca 2+分别以不同的速率扩散到细胞核或细胞质中，并影响这些区域的Ca 2+释放。(c)2010年，美国光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）[DOI电话：10.1117/1.3485741]
Calcium is an important messenger in cells and whose store and diffusion dynamics at the subcellular level remain unclear. By inducing a controlled slow subcellular Ca2+ release through femtosecond laser irradiation in HeLa cells immersed in different media, cytoplasm is identified to be the major intracellular Ca2+ store, with the nucleus being the minor store and the extracellular Ca2+ also contributing to the total cellular Ca2+ level. Furthermore, Ca2+ released in either the cytoplasm or nucleus diffuses into the nucleus or cytoplasm, respectively, at different rates and influences the Ca2+ release in those regions. (c) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3485741]