High degree polymerized chitooligosaccharides synthesis by chitosanase in the bulk aqueous system and reversed micellar microreactors
High degree polymerized chitooligosaccharides synthesis by chitosanase in the bulk aqueous system and reversed micellar microreactors
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DOI：
10.1016/j.procbio.2007.10.017
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发表时间：
2008-01-01
影响因子：
4.4
通讯作者：
Chiang, Been-Huang
中科院分区：
文献类型：
--
作者：
Hsiao, Yi-Chien;Lin, Yu-Wei;Chiang, Been-Huang
利用蜡状芽孢杆菌NTU-FC-4的壳聚糖酶生产高聚合度(DP)壳低聚糖。在水体系中，壳聚糖酶能够催化反应从二聚体、三聚体组成的混合物中生成少量的八聚体。四聚体、五聚体、六聚体和七聚体，可能通过糖基化反应。为了加强可能的转糖基化反应，在AOT(双-乙基己基)琥珀酸磺酸钠在异辛烷中形成的反胶束微反应器中进行了该过程，显著增加了高聚合度甲壳低聚糖的形成。结果表明，反胶束中的水分含量是影响酶促反应的重要因素。当水与表面活性剂(WO)的摩尔比为11.86时，反应产物中七聚体、八聚体和二十二聚体的含量最高。壳聚糖酶对纯壳低聚糖的酶解表明，五聚体和六聚体可能是酶反应中主要的糖基受体。(C)2007爱思唯尔有限公司。保留所有权利。
The chitosanase of Bacillus cereus NTU-FC-4 was used for the production of high degree polymerized (DP) chitooligosaccharides. In the aqueous system, the chitosanase was found to be able to catalyze a reaction to yield small amount of octamer from a mixture consisting of dimer, trimer. tetramer, pentamer, hexamer and heptamer, possibly through the transglycosylation reaction. To enhance the possible transglycosylation reaction, the process was carried out in the reversed micellar microreactors formed by AOT (sodium bis-2-(ethylhexyl) sulfosuccinate) in isooctane, and the formation of high DP chitooligosaccharides was significantly increased. It was found that the water content in the reverse micelles was an important factor affecting the enzymatic reaction. When the molar ratio of water to surfactant (WO) was 11.86, the reaction yielded the highest amount of heptamer, octamer, and nonamer. Hydrolysis of pure chitooligosaccharides by the chitosanase revealed that pentamer and hexamer might be the main gylcosyl acceptors during enzymatic reaction. (c) 2007 Elsevier Ltd. All rights reserved.