Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows
Field-Free Isolation of Exosomes from Extracellular Vesicles by Microfluidic Viscoelastic Flows
复制标题
通过微流体粘弹性流从细胞外囊泡中无场分离外泌体
DOI：
10.1021/acsnano.7b02277
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发表时间：
2017-07-01
期刊：
影响因子：
17.1
通讯作者：
Sun, Jiashu
中科院分区：
文献类型：
--
作者：
Liu, Chao;Guo, Jiayi;Sun, Jiashu
外泌体是几乎所有哺乳动物细胞分泌的分子货物，被认为是识别包括癌症在内的许多疾病的有希望的生物标志物。然而，外泌体的小尺寸（30-200 nm）在其从含有各种不同尺寸的细胞外囊泡（EV）的复杂培养基中分离时构成了严重的挑战，特别是在小样品体积中。在这里，我们提出了一种基于粘弹性的微流体系统，以连续的、尺寸依赖性的和无标记的方式从细胞培养基或血清中直接分离外泌体。使用少量的生物相容性聚合物作为介质中的添加剂来控制施加在EV上的粘弹力，我们能够实现外泌体的高分离纯度（>90%）和回收率（>80%）。所提出的技术可以作为一个通用的平台，以促进外来体分析在不同的生化应用。
Exosomes, molecular cargos secreted by almost all mammalian cells, are considered as promising biomarkers to identify many diseases including cancers. However, the small size of exosomes (30-200 nm) poses serious challenges in their isolation from complex media containing a variety of extracellular vesicles (EVs) of different sizes, especially in small sample volumes. Here we present a viscoelasticity-based microfluidic system to directly separate exosomes from cell culture media or serum in a continuous, size -dependent, and label -free manner. Using a small amount of biocompatible polymer as the additive in the media to control the viscoelastic forces exerted on EVs, we are able to achieve a high separation purity (>90%) and recovery (>80%) of exosomes. The proposed technique may serve as a versatile platform to facilitate exosome analyses in diverse biochemical applications.