Fate and plasticity of the endoderm in the early chick embryo
Fate and plasticity of the endoderm in the early chick embryo
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DOI：
10.1016/j.ydbio.2005.09.009
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发表时间：
2006-01-15
影响因子：
2.7
通讯作者：
Fukuda, K
中科院分区：
文献类型：
--
作者：
Kimura, W;Yasugi, S;Fukuda, K
在脊椎动物中，内胚层在原肠胚形成期间建立，并逐渐区域化为用于不同器官的区域。在这里，我们提出了精确的命运地图的原肠胚阶段鸡内胚层，使用的方法，旨在标记细胞特异性在较低的层。我们发现，第一个人口的内胚层细胞进入下层只有助于中肠和后肠;下一个细胞的入口有助于背侧前肠，最后由假定的腹侧前肠内胚层。移植实验表明，一些迁移的内胚层细胞，包括假定的腹前肠，入口从亨森氏结，而不是直接进入下层，而是迁移后的中间层内的一段距离。细胞移植表明，细胞在中间层已经致力于中胚层或内胚层，而细胞在原条是可塑的。基于这些结果，我们提出了一个修订的命运地图的位置和运动的前瞻性定形内胚层细胞在原肠胚形成。(c)2005年爱思唯尔公司All rights reserved.
In vertebrates, the endoderm is established during gastrulation and gradually becomes regionalized into domains destined for different organs. Here, we present precise fate maps of the gastrulation stage chick endoderm, using a method designed to label cells specifically in the lower layer. We show that the first population of endodermal cells to enter the lower layer contributes only to the midgut and hindgut; the next cells to ingress contribute to the dorsal foregut and followed finally by the presumptive ventral foregut endoderm. Grafting experiments show that some migrating endodermal cells, including the presumptive ventral foregut, ingress from Hensen's node, not directly into the lower layer but rather after migrating some distance within the middle layer. Cell transplantation reveals that cells in the middle layer are already committed to mesoderm or endoderm, whereas cells in the primitive streak are plastic. Based on these results, we present a revised fate map of the locations and movements of prospective definitive endoderm cells during gastrulation. (c) 2005 Elsevier Inc. All rights reserved.