TAL Effector DNA-Binding Principles and Specificity.
TAL Effector DNA-Binding Principles and Specificity.
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DOI：
10.1007/978-1-4939-2932-0_2
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发表时间：
2016
影响因子：
--
通讯作者：
Annekatrin Richter;Jana Streubel;J. Boch
中科院分区：
文献类型：
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作者：
Annekatrin Richter;Jana Streubel;J. Boch
转录激活因子样效应子（TALE）是具有独特DNA结合结构域的蛋白质，其赋予可预测和可编程的特异性。DNA结合结构域通常由34个氨基酸的近似相同的重复序列组成。重复序列形成右旋超螺旋结构，其缠绕在DNA双螺旋周围，并将每个重复序列的位置13处的可变氨基酸暴露于有义链DNA碱基。每个重复序列以高度特异性、非重叠和无逗号的方式结合一个碱基。虽然TALE特异性以简单的方式编码，但可以考虑复杂的规则来构建用于生物技术用途的高效DNA结合模块。
Transcription activator-like effectors (TALEs) are proteins with a unique DNA-binding domain that confers both a predictable and programmable specificity. The DNA-binding domain consists typically of 34-amino acid near-identical repeats. The repeats form a right-handed superhelical structure that wraps around the DNA double helix and exposes the variable amino acids at position 13 of each repeat to the sense strand DNA bases. Each repeat binds one base in a highly specific, non-overlapping, and comma-free fashion. Although TALE specificities are encoded in a simple way, sophisticated rules can be taken into account to build highly efficient DNA-binding modules for biotechnological use.