REPLICATION AND RECOMBINATION FUNCTIONS ASSOCIATED WITH THE YEAST PLASMID, 2-MU CIRCLE
REPLICATION AND RECOMBINATION FUNCTIONS ASSOCIATED WITH THE YEAST PLASMID, 2-MU CIRCLE
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DOI：
10.1016/0092-8674(80)90487-0
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发表时间：
1980-01-01
期刊：
影响因子：
64.5
通讯作者：
HICKS, JB
中科院分区：
文献类型：
--
作者：
BROACH, JR;HICKS, JB
通过检查含有2 μ m特定区域的各种质粒的酵母转化效率，环基因组和所得转化体的特征，2 μ C的几个区域，环基因组，其参与2 μ。鉴定了环状复制或重组。通过鉴定来自促进酵母高频转化的分子的那些DNA片段，将复制起点定位于部分位于大的独特区域内的序列，如通过随后的缺失分析所确定的，所述大的独特区域从反向重复区域的中间延伸到连续的独特区域中。通过检测含有完整的2 μ M质粒或2 μ M质粒的杂合质粒在酵母中的相对复制效率，环状基因组或2 μ l的片段。包围复制起点的圆，确定有效利用2 μ m的DNA片段是有效的。环原点需要在远离原点的位点处的分子中编码的一些功能。通过检测突变体2 μ.环状分子在酵母中进行分子内重组，将2 μ鉴定了编码该过程所需产物的circle基因。
By examining both the transformation efficiency of yeast of various plasmids containing defined regions of the 2.mu. circle genome and the characteristics of the resultant transformants, several regions of the 2 .mu. circle genome which are involved in 2 .mu. circle replication or recombination were identified. By identifying those DNA fragments from the molecule which promote high frequency transformation of yeast, the origin of replication to a sequence partially within the large unique region was localized which, as determined by subsequent deletion analysis, extends from the middle of the inverted repeat region into the contiguous unique region. By examining the relative efficiency of replication in yeast of hybrid plasmids containing either the entire 2.mu. circle genome or a fragment of 2.mu. circle encompassing the origin of replication, it was determined that efficient use of the 2.mu. circle origin requires some function or functions encoded in the molecule at a site away from the origin. By examining the ability of a mutant 2.mu. circle molecule to undergo intramolecular recombination in yeast, a 2.mu. circle gene which codes for a product required for this process was identified.