Impact of a novelRickettsiasymbiont on the life history and virus transmission capacity of its host whitefly (Bemisia tabaci)
Impact of a novelRickettsiasymbiont on the life history and virus transmission capacity of its host whitefly (Bemisia tabaci)
复制标题
一种新型立克次体共生体对其宿主烟粉虱（烟粉虱）生活史和病毒传播能力的影响
DOI：
10.1111/1744-7917.12797
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发表时间：
2020
期刊：
影响因子：
4
通讯作者：
Liu Shu-Sheng
中科院分区：
文献类型：
--
作者：
Lei Teng;Zhao Jing;Wang Hua-Ling;Liu Yin-Quan;Liu Shu-Sheng
立克次体是昆虫中最常见的共生体，在宿主生物学中起着重要作用。最近，在烟粉虱Bemisia tabacicomplex中发现了一个由母系遗传的Torix群立克次体Rickettsia，暂定名为RiTBt。这种立克次体在宿主生物学中的作用尚不清楚。在这里，我们研究了RiTBt对Asia II 1的性能和病毒传播能力的影响。RiTBt对烟粉虱生活史参数的影响不显著，但在烟草、番茄和棉花上饲养时，RiTBt对烟粉虱生活史参数的影响不显著。有趣的是，RiTBt略微增强了粉虱传播棉花卷叶木尔坦病毒（CLCuMuV），这是一种由粉虱在田间传播的病毒，对棉花生产造成了广泛的损害。具体而言，与没有RiTBt的粉虱相比，在48小时病毒采集后，具有RiTBt的粉虱具有更高的病毒滴度，并显示出更高的病毒传播效率。立克次体分泌蛋白BtR242被鉴定为推定的病毒结合蛋白，并观察到与CLCuMuVin的外壳蛋白体外相互作用。病毒感染的粉虱下调基因转录水平的BtR242基因。这些观察结果表明，RiTBt对亚洲II 1粉虱生物学的影响有限，这种共生体是否在其他宿主粉虱生物学中具有功能，值得进一步研究。
Rickettsiaconsists of some of the most prevalent symbionts of insects and often plays a significant role in the biology of its hosts. Recently, a maternally inherited Torix groupRickettsia, provisionally named as RiTBt, was recorded in a species of notorious pest whitefly, tentatively named as Asia II 1, from theBemisia tabacicomplex. The role of thisRickettsiain the biology of its host is unknown. Here we investigated the impact of RiTBt on the performance and virus transmission capacity of Asia II 1. RiTBt did not significantly affect the life history parameters of the whitefly when the host insect was reared on tobacco, tomato, and cotton, three host plants with relatively low, medium and high suitability to the whitefly. Intriguingly, RiTBt slightly enhanced whitefly transmission of cotton leaf curl Multan virus (CLCuMuV), a virus that is transmitted by the whitefly in the field and has caused extensive damage to cotton production. Specifically, compared with whiteflies without RiTBt, following a 48 h virus acquisition whiteflies with RiTBt had higher titer of virus and showed higher efficiency of virus transmission. A rickettsial secretory protein BtR242 was identified as a putative virus‐binding protein, and was observed to interact with the coat protein of CLCuMuVin vitro. Viral infection of the whitefly downregulated gene transcript levels of theBtR242gene. These observations indicate that RiTBt has limited impact on the biology of the Asia II 1 whitefly, and whether this symbiont has functions in the biology of other host whiteflies warrants future investigation.