Correlation between copy number of mitochondrial DNA and clinico-pathologic parameters of hepatocellular carcinoma
Correlation between copy number of mitochondrial DNA and clinico-pathologic parameters of hepatocellular carcinoma
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DOI：
10.1016/j.ejso.2006.01.002
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发表时间：
2006-04-01
期刊：
影响因子：
3.8
通讯作者：
Nakao, A
中科院分区：
文献类型：
--
作者：
Yamada, S;Nomoto, S;Nakao, A
目的：方法：采用实时荧光定量聚合酶链反应（RT-PCR）技术对31例肝细胞癌（HCC）标本进行检测，分析其mtDNA拷贝数与临床病理参数及线粒体基因组D-loop区突变的相关性。与相应的非癌肝组织相比，HCC中的mtDNA拷贝数减少（p=0.002），并且与肿瘤大小（p=0.014）和肝硬化（p=0.048）显著相关。当通过Kaplan-Meier曲线评估时，mtDNA拷贝数低的患者倾向于显示比mtDNA拷贝数高的患者更短的5年生存率，但不显著（总生存率，63 vs 83%; p=0.19）。mtDNA D-loop突变或缺失的肝癌细胞mtDNA拷贝数较低，但差异无统计学意义（分别为p=0.656，p = 0.590）。(c)2006爱思唯尔有限公司保留所有权利。
Aims: In the current study, we investigated possible correlations of the mtDNA copy number in hepatocellular carcinoma (HCC) with the pathological findings and prognosis.Methods: We studied 31 HCC specimens using quantitative real-time polymerase chain reaction analysis, and the correlation between the mtDNA copy number and the clinicopathologic parameters and mutations in the D-loop region of the mitochondrial genome.Results: The mtDNA copy number was reduced in HCCs compared with the corresponding non-cancerous liver tissues (p=0.002), and significantly correlated with tumour size (p=0.014) and cirrhosis (p=0.048). Patients with a low mtDNA copy number tended to show shorter 5-year Survival rates than patients with a high mtDNA copy number when assessed by Kaplan-Meier curves, but not a significant (overall survival rate, 63 vs 83%; p=0.19). The copy number of HCC with mtDNA D-loop mutation or deletion was lower, but not significantly so (p=0.656, p = 0.590, respectively).Conclusions: Our results indicated that it reduced copy number of mtDNA is correlated with HCC and associated with malignant potential. (c) 2006 Elsevier Ltd. All rights reserved.