ANALYSIS OF PERIOD MESSENGER-RNA CYCLING IN DROSOPHILA HEAD AND BODY-TISSUES INDICATES THAT BODY OSCILLATORS BEHAVE DIFFERENTLY FROM HEAD OSCILLATORS
ANALYSIS OF PERIOD MESSENGER-RNA CYCLING IN DROSOPHILA HEAD AND BODY-TISSUES INDICATES THAT BODY OSCILLATORS BEHAVE DIFFERENTLY FROM HEAD OSCILLATORS
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DOI：
10.1128/mcb.14.11.7211
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发表时间：
1994-11-01
影响因子：
5.3
通讯作者：
HARDIN, PE
中科院分区：
文献类型：
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作者：
HARDIN, PE
period（per）基因被认为是果蝇昼夜节律起搏器的一部分。构成每反馈回路的每RNA和蛋白质的昼夜节律波动似乎是起搏器功能所需的，并且已经在运动活动和羽化节律所必需的头部神经元组织中进行了测量。Per基因也在许多神经元和非神经元的身体组织中表达，对于这些组织还没有描述过已知的昼夜节律现象。为了确定per是否可能影响这些身体组织中的一些昼夜节律功能，检查了per RNA循环。这些研究表明，在光暗周期中，头部和身体组织中的每RNA周期处于相同的相位和幅度。一个例外是卵巢中缺乏PER RNA循环，这似乎也是PER蛋白主要在细胞质中的唯一组织。然而，在持续的黑暗中，每个RNA循环的幅度在身体中比在头部中衰减得更快。两者合计，这些结果表明，昼夜节律振荡器存在于头部和身体组织中，其中PER蛋白是核，并且这些振荡器的行为不同。
The period (per) gene is thought to be part of the Drosophila circadian pacemaker. The circadian fluctuations in per RNA and protein that constitute the per feedback loop appear to be required for pacemaker function, and have been measured in head neuronal tissues that are necessary for locomotor activity and eclosion rhythms. The per gene is also expressed in a number of neuronal and nonneuronal body tissues for which no known circadian phenomena have been described. To determine whether per might affect some circadian function in these body tissues, per RNA cycling was examined. These studies show that per RNA cycles in the same phase and amplitude in head and body tissues during light-dark cycles. One exception to this is the lack of per RNA cycling in the ovary, which also appears to be the only tissue in which PER protein is primarily cytoplasmic. In constant darkness, however, the amplitude of per RNA cycling dampens much more quickly in bodies than in heads. Taken together, these results indicate that circadian oscillators are present in head and body tissues in which PER protein is nuclear and that these oscillators behave differently.