Alteration of the fecal microbiota in Chinese patients with Parkinson's disease
Alteration of the fecal microbiota in Chinese patients with Parkinson's disease
复制标题
中国帕金森病患者粪便微生物群的变化
DOI：
10.1016/j.bbi.2018.02.016
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发表时间：
2018-05-01
影响因子：
15.1
通讯作者：
Xiao, Qin
中科院分区：
文献类型：
--
作者：
Qian, Yiwei;Yang, Xiaodong;Xiao, Qin
新出现的证据表明，肠道微生物群失调在帕金森病（PD）中发挥作用。然而，中国PD患者粪便微生物组的变化仍不清楚。本病例对照研究旨在探索中国PD患者的粪便微生物群组成。使用针对16 S核糖体RNA（rRNA）基因V3-V4区域的高通量Illumina Miseq测序，研究了45名患者及其健康配偶粪便中的微生物群。分析粪便菌群与PD临床特征的关系。PD患者和健康对照组之间粪便微生物群的结构和丰富度不同。在调整年龄、性别、体重指数（BMI）和便秘后，PD患者粪便中富含梭菌IV属、水杆菌属、霍德曼氏菌属、鞘氨醇单胞菌属、梭菌XVIII属、丁酸球菌属和厌氧干球菌属。此外，埃希氏菌/志贺菌属与病程呈负相关。Dorea和Phascolarctobacterium与左旋多巴当量剂量（LED）呈负相关。在非运动症状（NMS）中，丁酸菌属和梭菌属XIVb与认知障碍相关。总体而言，我们证实了中国PD患者存在肠道微生物群失调。一个控制良好的人群参与有利于识别与疾病相关的微生物群。此外，粪便菌群与PD临床特征密切相关。阐明粪便微生物组中的这些差异将为改善我们对PD发病机制的理解提供基础，并支持改变肠道微生物群的潜在治疗方案。(C)2018爱思唯尔公司All rights reserved.
Emerging evidences suggest that gut microbiota dysbiosis plays a role in Parkinson's disease (PD). However, the alterations in fecal microbiome in Chinese PD patients remains unknown. This case control study was conducted to explore fecal microbiota compositions in Chinese PD patients. Microbiota communities in the feces of 45 patients and their healthy spouses were investigated using high-throughput Illumina Miseq sequencing targeting the V3-V4 region of 16S ribosomal RNA (rRNA) gene. The relationships between fecal microbiota and PD clinical characteristics were analyzed. The structure and richness of the fecal microbiota differed between PD patients and healthy controls. Genera Clostridium IV, Aquabacterium, Holdemania, Sphingomonas, Clostridium XVIII, Butyricicoccus and Anaerotruncus were enriched in the feces of PD patients after adjusting for age, gender, body mass index (BMI), and constipation. Furthermore, genera Escherichia/Shigella were negatively associated with disease duration. Genera Dorea and Phascolarctobacterium were negatively associated with levodopa equivalent doses (LED). Among the non-motor symptoms (NMSs), genera Butyricicoccus and Clostridium XIVb were associated with cognitive impairment. Overall, we confirmed that gut microbiota dysbiosis occurs in Chinese patients with PD. A well-controlled population involved was beneficial for the identification of microbiota associated with diseases. Additionally, the fecal microbiota was closely related to PD clinical characteristics. Elucidating these differences in the fecal microbiome will provide a foundation to improve our understanding the pathogenesis of PD and to support the potentially therapeutic options modifying the gut microbiota. (C) 2018 Elsevier Inc. All rights reserved.