Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions.
Induction of Meiotic Initiation in Long-Term Mouse Spermatogonial Stem Cells Under Retinoid Acid and Nutrient Restriction Conditions.
复制标题
在视黄酸和营养限制条件下诱导长期小鼠精原干细胞减数分裂起始。
DOI：
10.1007/978-1-0716-3698-5_9
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发表时间：
2024
期刊：
影响因子：
--
通讯作者：
Wang,Ning
中科院分区：
文献类型：
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作者：
Zhang,Xiaoyu;Wang,Ning
精原干细胞（spermatogonialstemcells，SSCs）在体内通过有丝分裂和减数分裂产生单倍体精子。尽管培养小鼠精原干细胞的技术已经很成熟，但体外诱导减数分裂仍然是一个挑战。视黄酸（RA）是体内减数分裂所必需的;然而，单独的RA不足以在体外诱导减数分裂。在这里，我们描述了一种方法，其中营养限制和RA协同诱导减数分裂开始进入减数分裂前期I培养的小鼠精原干细胞。
Spermatogonial stem cells (SSCs) produce haploid sperm via mitosis and meiosis in vivo. Although the technique to culture mouse SSCs has been well established, induction of meiosis in vitro has remained a challenge. Retinoic acid (RA) is required for meiosis in vivo; however, RA alone is not sufficient to induce meiosis in vitro. Here, we describe a method in which nutrient restriction and RA synergistically induce meiotic initiation into meiotic prophase I in cultured mouse SSCs.