Secondary structure prediction of 52 membrane-bound cytochromes P450 shows a strong structural similarity to P450cam.
Secondary structure prediction of 52 membrane-bound cytochromes P450 shows a strong structural similarity to P450cam.
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52 种膜结合细胞色素 P450 的二级结构预测显示与 P450cam 具有很强的结构相似性。
DOI：
10.1021/bi00428a036
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发表时间：
1989
期刊：
影响因子：
2.9
通讯作者：
Strobel,HW
中科院分区：
文献类型：
--
作者：
Nelson,DR;Strobel,HW
修订稿于 1988 年 8 月 30 日收到 摘要：预测了 52 个对齐的细胞色素 P450 序列的二级结构，所有这些序列都是膜结合的，并与可溶性细胞色素 P450eam 的晶体结构进行了共同比较。预计膜结合的 P450 中存在 13 个螺旋区域中的 10 个、7/3 对区域中的 6 个和与 P450cam 活性位点附近已知的/3-凸起相对应的/3-结构。对于膜结合形式，还预测了与可溶性酶中的/3-结构相关的三个转角。膜 P450s 和可溶性 P450cam 之间存在明显的结构相似性。因此，涉及大部分 P450 的多跨膜结构似乎不太可能。具有两个 N 端跨膜片段的结构与这些观察相一致。（细胞色素 P450 指的是激活分子氧的多种 6 型细胞色素。然后活化的氧与许多亲脂性底物原位反应（Ortiz de Montellano，1986；Black & Coon，1987）。人们对这些酶的结构和功能非常感兴趣。自 1982 年以来，超过已发表 100 个 P450 序列，代表至少 53 个独特的 P450 蛋白
Revised Manuscript Received August 30, 1988 abstract: The secondary structure of 52 aligned cytochrome P450 sequences, all of which are membrane bound, is predicted and collectively compared with the crystal structure of the soluble cytochrome P450eam. Ten of 13 helical regions, 6 of 7/3-pair regions, and/3-structure corresponding to a known/3-bulge near the active site of P450cam are predicted to exist in the membrane-bound P450s. Three turns associated with/3-structure in the soluble enzyme are also predicted for the membrane-bound forms. A strong structural similarity is evident between membrane P450s and the soluble P450cam. Consequently, a multitransmembrane structure involving much of P450 seems highly unlikely. A structure with two N-terminal transmembrane segments is compatible with these observations.(Cytochrome P450 designates a diverse class of 6-type cy-tochromes that activate molecular oxygen. The activated oxygen then reacts in situ with many lipophilic substrates (Ortiz de Montellano, 1986; Black & Coon, 1987). There is considerable interest in the structure and function of these enzymes. Since 1982, over 100 P450 sequences have been published, representing at least 53 unique P450 proteins and