Three types of acetylcholine-induced single channel currents in clonal rat pheochromocytoma cells
Three types of acetylcholine-induced single channel currents in clonal rat pheochromocytoma cells
复制标题
克隆大鼠嗜铬细胞瘤细胞中乙酰胆碱诱导的三种类型的单通道电流
DOI：
10.1016/0304-3940(83)90301-4
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发表时间：
1983
影响因子：
2.5
通讯作者：
H. Matthaei
中科院分区：
文献类型：
--
作者：
J. Bormann;H. Matthaei
应用膜片钳技术，在神经生长因子分化的克隆性大鼠嗜铬细胞瘤（PC 12）细胞上记录乙酰胆碱诱导的单通道电流。内向电流的幅度是不均匀的，可能与三类单位电流。对于膜片膜电位范围为− 50 mV至− 120 mV，三种电流-电压关系呈线性。在22°C下，获得22 pS、31 pS和39 pS的电导。通道的外推反转电位为0 mV，平均开放时间约为10-20 ms。这些结果可能表明，PC 12细胞具有三种类型的乙酰胆碱受体通道。
Single channel currents induced by acetylcholine were recorded from nerve growth factor-differentiated clonal rat pheochromocytoma (PC12) cells by using the patch-clamp technique. Inward current amplitudes were heterogeneous and could be related to three classes of unitary current. The three current-voltage relations were linear for patch membrane potentials ranging from − 50 mV to − 120 mV. At 22°C, conductances of 22 pS, 31 pS and 39 pS were obtained. The extrapolated reversal potential for the channels was 0 mV and the mean open time approximately 10–20 ms. These results may indicate that PC12 cells possess three types of acetylcholine receptor channels.