A comparative study of the interaction of two structurally analogous ruthenium complexes with human telomeric G-quadruplex DNA.
A comparative study of the interaction of two structurally analogous ruthenium complexes with human telomeric G-quadruplex DNA.
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DOI：
10.1016/j.jinorgbio.2012.12.011
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发表时间：
2013-04
影响因子：
3.9
通讯作者：
Shuo Shi;Hai-Liang Huang;Xing Gao;Junliang Yao;ChunSheng Lv;Juan Zhao;Wen-Liang Sun;Tianming Yao-Tian
中科院分区：
文献类型：
--
作者：
Shuo Shi;Hai-Liang Huang;Xing Gao;Junliang Yao;ChunSheng Lv;Juan Zhao;Wen-Liang Sun;Tianming Yao-Tian
Two new polypyridine ligands and their corresponding ruthenium(II) complexes have been prepared and characterized. The interactions of both complexes with human telomere quadruplex DNA (both the antiparallel basket and the mixed-hybrid G-quadruplex) have been studied by circular dichroism (CD), CD melting, UV-visible (UV-Vis), fluorescent intercalator displacement (FID) assays and molecular docking studies. The results show that both complexes can stabilize G-quadruplexes DNA and two complexes show different binding affinity for different G-quadruplexes DNA. The 1:1 stoichiometry was confirmed in the buffered solutions by the UV-Vis spectrophotometer using Job's plot method and molecular docking studies. We have also investigated the interaction between the complexes and duplex DNA to gain some insight into the selectivity of the complexes for G-quadruplex structures. FID studies have shown that the complexes have a modest selectivity for G-quadruplex versus duplex DNA.
