H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China
H9N2 influenza viruses possessing H5N1-like internal genomes continue to circulate in poultry in southeastern China
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DOI：
10.1128/jvi.74.20.9372-9380.2000
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发表时间：
2000-10-01
影响因子：
5.4
通讯作者：
Peiris, M
中科院分区：
文献类型：
--
作者：
Guan, Y;Shortridge, KF;Peiris, M
H9N2流感病毒向人类传播，以及认识到中国东南部H9N2病毒中可能存在A/Hong Kong/156/97样（H5N1）（简称HK/156/97）基因组复合体，有必要对1999年香港特别行政区家禽中H9N2病毒的分布和特征进行研究。血清学研究表明，H9N2流感病毒感染了中国东南部大量的鸡和其他陆生鸟类（鸽子、野鸡、鹌鹑、珍珠鸡和家鸡）。在活禽市场发现的两种H9N2流感病毒为A/鹌鹑/香港/G1/97 （Qa/HK/G1/97）样病毒和A/鸭/香港/Y280/97 （Dk/HK/Y280/97）样病毒。在家禽市场上，高达16%的鹌鹑笼含有Qa/HK/ g1 /97样病毒，而其他陆生禽类笼中约有5%感染Dk/HK/ y280 /97样病毒。尽管两种H9N2病毒谱系在家禽市场上共同流通，但未发现它们之间的重组。以A/Chicken/Hong Kong/G9/97 （H9N2）为代表的重组病毒是1997年在香港市场流行的主要H9N2流感病毒，但自1997年宰鸡事件后再未检出。Qa/HK/ g1 /97样病毒多见于鹌鹑，Dk/HK/ y280 /97样病毒主要见于鸡。Qa/HK/ g1 /97样病毒进化速度较快，特别是PB2、HA、NP和NA基因的进化速度较快，提示它们正在适应新的宿主。实验研究表明，两种H9N2谱系主要通过气溶胶途径传播，鹌鹑和鸡均未显示出患病的证据。鹌鹑感染含有与HK/156/97 （H5N1）病毒遗传高度相关的6个基因片段的Qa/HK/ g1 /97样病毒的高流行率强调了对包括人类在内的哺乳动物进行监测的必要性。
The transmission of H9N2 influenza viruses to humans and the realization that the A/Hong Kong/156/97-like (H5N1) (abbreviated HK/156/97) genome complex may be present in H9N2 viruses in southeastern China necessitated a study of the distribution and characterization of H9N2 viruses in poultry in the Hong Kong SAR in 1999. Serological studies indicated that H9N2 influenza viruses had infected a high proportion of chickens and other land-based birds (pigeon, pheasant, quail, guinea fowl, and chukka) from southeastern China. Two lineages of H9N2 influenza viruses present in the live-poultry markets were represented by A/Quail/Hong Kong/G1/97 (Qa/HK/G1/97)-like and A/Duck/Hong Kong/Y280/97 (Dk/HK/Y280/97)-like viruses. Up to 16% of cages of quail in the poultry markets contained Qa/HK/G1/97-like viruses, while about 5% of cages of other land-based birds were infected with Dk/HK/Y280/97-like viruses. No reassortant between the two H9N2 virus Lineages was detected despite their cocirculation in the poultry markets. Reassortant viruses represented by A/Chicken/Hong Kong/G9/97 (H9N2) were the major H9N2 influenza viruses circulating in the Hong Kong markets in 1997 but have not been detected since the chicken slaughter in 1997. The Qa/HK/G1/97-like viruses were frequently isolated from quail, while Dk/HK/Y280/97-like viruses were predominately associated with chickens. The Qa/HK/G1/97-like viruses were evolving relatively rapidly, especially in their PB2, HA, NP, and NA genes, suggesting that they are in the process of adapting to a new host. Experimental studies showed that both H9N2 lineages were primarily spread by the aerosol route and that neither quail nor chickens showed evidence of disease. The high prevalence of quail infected with Qa/HK/G1/97-like virus that contains six gene segments genetically highly related to HK/156/97 (H5N1) virus emphasizes the need for surveillance of mammals including humans.