RETROVIRAL VECTOR SYSTEM FOR THE STUDY OF CDNA GENE FORMATION
RETROVIRAL VECTOR SYSTEM FOR THE STUDY OF CDNA GENE FORMATION
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DOI：
10.1128/mcb.8.6.2328
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发表时间：
1988-06-01
影响因子：
5.3
通讯作者：
TEMIN, HM
中科院分区：
文献类型：
--
作者：
DORNBURG, R;TEMIN, HM
开发了逆转录病毒载体系统以研究缺乏正常逆转录病毒复制所需的所有顺式作用序列的RNA的逆转录转座。我们的实验表明，这样的RNA可以在逆转录病毒蛋白中被固定化，逆转录，并在感染的细胞中整合形成功能性cDNA基因。然而，这一过程的频率比正常逆转录病毒复制的频率低约8个数量级。在这个过程中，每一步的效率都是有限的。对7个cDNA基因的Southern杂交分析表明，它们都在3“或5”端或两者都被截短。这些截短在天然cDNA基因中是看不到的，并提出了逆转录病毒参与其形成的问题。
A retroviral vector system was developed to study the retrotransposition of RNAs lacking all cis-acting sequences required for normal retroviral replication. Our experiments indicate that such RNAs can be encapsidated in retroviral proteins, reverse transcribed, and integrated to form functional cDNA genes in infected cells. The frequency of this process, however, was approximately 8 orders of magnitude less than that of normal retroviral replication. The efficiency was limited at each step in this process. Investigation of seven cDNA genes by Southern blot analysis revealed that all of them were truncated at either the 3'' or the 5'' end or both. These truncations are not seen with natural cDNA genes and raise the question of retroviral involvement in their formation.