Exploring surface characterization and electrostatic property of Hybrid Pennisetum during alkaline sulfite pretreatment for enhanced enzymatic hydrolysability
Exploring surface characterization and electrostatic property of Hybrid Pennisetum during alkaline sulfite pretreatment for enhanced enzymatic hydrolysability
复制标题
探索杂交狼尾草在碱性亚硫酸盐预处理过程中的表面特征和静电特性，以增强酶水解能力
DOI：
10.1016/j.biortech.2017.08.046
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发表时间：
2017
影响因子：
11.4
通讯作者：
Zhang Junhua
中科院分区：
文献类型：
--
作者：
Yang Ming;Wang Jingfeng;Hou Xincun;Wu Juying;Fan Xifeng;Jiang Fan;Tao Pan;Wang Fan;Peng Pai;Yang Fangxia;Zhang Junhua
研究了杂种狼尾草（HP）经碱性亚硫酸盐预处理后的表面表征和静电性能，以提高其酶解能力。HP的O/C比值从0.34增加到0.60，C1浓度从62.5%下降到31.6%，说明碱性亚硫酸盐预处理使HP表面木质素减少，碳水化合物增加。Zeta电位和硫元素分析表明，碱性亚硫酸盐预处理HP的碳水化合物表面由于木质素磺化，有利于更多的酶吸附，从而减少了非生产性吸附。在纤维素酶和木聚糖酶的协同作用下，碱性亚硫酸盐预处理HP的葡萄糖产率达到100%，显著高于NaOH预处理，葡萄糖释放浓度提高1.52倍。结果表明，碱性亚硫酸盐预处理具有提高HP水解率的潜力，其表面表征和静电特性有利于酶解。
The surface characterization and electrostatic property of Hybrid Pennisetum (HP) after alkaline sulfite pretreatment were explored for enhanced enzymatic hydrolysability. The O/C ratio in HP increased from 0.34 to 0.60, and C1 concentration decreased from 62.5% to 31.6%, indicating that alkaline sulfite pretreatment caused poorer lignin but richer carbohydrate on HP surface. Zeta potential and sulfur element analysis indicated that more enzymes would preferably adsorb on the carbohydrate surface of alkaline sulfite pretreated HP because the lignin was sulfonated, which facilitated the decrease of non-productive adsorption. Glucose yield of alkaline sulfite pretreated HP reached to 100% by synergistic action of cellulase and xylanase in the hydrolysis, which was significantly higher than that of NaOH pretreated, and the concentration of glucose released was 1.52 times higher. The results suggested that alkaline sulfite pretreatment had potential for improving the HP hydrolysability, and the surface characterization and electrostatic property facilitated the enzymatic digestibility.