Amino acid composition, including key derivatives of eccrine sweat: potential biomarkers of certain atopic skin conditions
Amino acid composition, including key derivatives of eccrine sweat: potential biomarkers of certain atopic skin conditions
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DOI：
10.1111/ics.12019
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发表时间：
2013-04-01
影响因子：
2.3
通讯作者：
Harding, Clive R.
中科院分区：
文献类型：
--
作者：
Mark, Harker;Harding, Clive R.
汗液的游离氨基酸（AA）组成与其他生物体液不同，原因尚不清楚。我们对新鲜分离的纯人汗液的AA成分进行了详细的分析，包括一些AA代谢的关键衍生物，以更好地了解其组成的关键生物学机制。12名健康受试者在形成后立即从腋窝收集汗液。茚三酮衍生化后，用自动AA分析仪进行游离AA分析。GC/MS法测定吡咯烷-5-羧酸（PCA）和尿酸（UCA）水平。汗液的游离AA组成以丝氨酸为主，占游离AA总组成的五分之一多一点。甘氨酸含量次之，其次是PCA、丙氨酸、瓜氨酸和苏氨酸。结果表明，汗液中的AA含量与表皮蛋白聚面蛋白的AA组成具有显著的相似性。该蛋白是角质层（SC）内天然保湿因子（NMF）的主要组成部分的游离aa及其衍生物的主要来源，在维持人体皮肤屏障完整性方面起着重要作用。由于NMF产生的扰动可导致屏障功能异常，并可能由于聚丝蛋白基因型而引起，因此我们提出汗液中AAs的定量可作为某些特应性皮肤疾病（即特应性皮炎（AD））的非侵入性诊断生物标志物。
Synopsis The free amino acid (AA) composition of eccrine sweat is different from other biological fluids, for reasons which are not properly understood. We undertook the detailed analysis of the AA composition of freshly isolated pure human eccrine sweat, including some of the key derivatives of AA metabolism, to better understand the key biological mechanisms governing its composition. Eccrine sweat was collected from the axillae of 12 healthy subjects immediately upon formation. Free AA analysis was performed using an automatic AA analyser after ninhydrin derivatization. Pyrrolidine-5-carboxylic acid (PCA) and urocanic acid (UCA) levels were determined using GC/MS. The free AA composition of sweat was dominated by the presence of serine accounting for just over one-fifth of the total free AA composition. Glycine was the next most abundant followed by PCA, alanine, citrulline and threonine, respectively. The data obtained indicate that the AA content of sweat bears a remarkable similarity to the AA composition of the epidermal protein profilaggrin. This protein is the key source of free AAs and their derivatives that form a major part of the natural moisturizing factor (NMF) within the stratum corneum (SC) and plays a major role in maintaining the barrier integrity of human skin. As perturbations in the production of NMF can lead to abnormal barrier function and can arise as a consequence of filaggrin genotype, we propose the quantification of AAs in sweat may serve as a non-invasive diagnostic biomarker for certain atopic skin conditions, that is, atopic dermatitis (AD).