Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering.: I:: preparation and in vitro characterisation
Porous poly(α-hydroxyacid)/Bioglass® composite scaffolds for bone tissue engineering.: I:: preparation and in vitro characterisation
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用于骨组织工程的多孔聚（α-羟基酸）/Bioglass®复合支架：I：：制备和体外表征
DOI：
10.1016/j.biomaterials.2003.10.082
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发表时间：
2004-08-01
期刊：
影响因子：
14
通讯作者：
Jérôme, R
中科院分区：
文献类型：
--
作者：
Maquet, V;Boccaccini, AR;Jérôme, R
通过热诱导固-液相分离（TIPS）和随后的溶剂升华制备含有不同量（10、25和50重量%）生物活性玻璃（45 S5 Bioglass（R））的聚D，L-丙交酯（PDLLA）和聚（丙交酯-共-乙交酯）（PLGA）的高度多孔复合材料支架。将增加量的生物玻璃（R）添加到聚合物泡沫中降低了孔体积。相反，聚合物材料的机械性能得到改善。将复合材料在37 ℃的磷酸盐缓冲盐水中孵育，通过测量吸水率、重量损失以及聚合物的平均分子量和孵育介质的pH值随孵育时间的变化来研究聚合物的体外降解。与纯聚合物泡沫相比，向聚合物泡沫中添加Bioglass（R）增加了吸水性和重量损失。然而，发现通过尺寸排阻色谱法测定的聚合物分子量在不存在Bioglass（R）的情况下降低得更快并且降低的程度更大。因此，发现与纯聚合物泡沫相比，生物活性填料的存在延迟了聚合物的降解速率。通过EDXA、X射线衍射和拉曼光谱等手段对复合材料表面羟基磷灰石的形成进行了表征。(C)2003 Elsevier Ltd.保留所有权利。
Highly porous composites scaffolds of Poly-D,L-lactide (PDLLA) and poly(lactide-co-glycolide) (PLGA) containing different amounts (10, 25 and 50 wt%) of bioactive glass (45S5 Bioglass(R)) were prepared by thermally induced solid-liquid phase separation (TIPS) and subsequent solvent sublimation. The addition of increasing amounts of Bioglass(R) into the polymer foams decreased the pore volume. Conversely, the mechanical properties of the polymer materials were improved. The composites were incubated in phosphate buffer saline at 37degreesC to study the in vitro degradation of the polymer by measurement of water absorption, weight loss as well as changes in the average molecular weight of the polymer and in the pH of the incubation medium as a function of the incubation time. The addition of Bioglass(R) to polymer foams increased the water absorption and weight loss compared to neat polymer foams. However, the polymer molecular weight, determined by size exclusion chromatography, was found to decrease more rapidly and to a larger extent in absence of Bioglass(R). The presence of the bioactive filler was therefore found to delay the degradation rate of the polymer as compared to the neat polymer foams. Formation of hydroxyapatite on the surface of composites, as an indication of their bioactivity, was recorded by EDXA, X-ray diffractometry and confirmed by Raman spectroscopy. (C) 2003 Elsevier Ltd. All rights reserved.