Effects of Exposure to Mild Hyperbaric Oxygen on DSS-Induced Colonic Inflammation and Diarrhea in Rats
Effects of Exposure to Mild Hyperbaric Oxygen on DSS-Induced Colonic Inflammation and Diarrhea in Rats
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DOI：
10.2147/jir.s220586
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发表时间：
2019-10
影响因子：
4.5
通讯作者：
A. Takemura;T. Egawa;Takuji Tanaka;T. Kuramoto;Tatsuya Hayashi;A. Ishihara
中科院分区：
文献类型：
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作者：
A. Takemura;T. Egawa;Takuji Tanaka;T. Kuramoto;Tatsuya Hayashi;A. Ishihara
目的在啮齿类动物中，葡聚糖硫酸钠（DSS）诱导的腹泻和结肠炎症具有与人类溃疡性结肠炎相似的症状。我们研究了暴露于轻度高压氧（MHO）在1317 hPa的大气压与40%的氧气对DSS诱导的腹泻和结肠炎症大鼠的影响。方法5周龄雄性京都Apc Delta（KAD）大鼠（n = 12）经饮水给予2%DSS 1周。随后，DSS处理的雄性大鼠不接受任何进一步处理（n = 6）或暴露于MHO（n = 6）2周。未进行DSS治疗或暴露于MHO的匹配KAD大鼠用作对照组（n = 6）。结果对照组大鼠未出现腹泻和结肠炎。然而，DSS治疗的大鼠表现出腹泻和结肠炎症，无论暴露于MHO。暴露于MHO 2周导致DSS治疗的大鼠中腹泻的发生率降低（p < 0.05）。暴露于MHO对DSS处理的大鼠的结肠炎症没有影响（p = 0.12）。结论MHO暴露2周可改善腹泻，但不能减轻结肠炎症，可能是由于本研究中使用的暴露时间较短（2周）。
Purpose In rodents, dextran sulfate sodium (DSS)-induced diarrhea and colonic inflammation have similar symptoms to those of ulcerative colitis in humans. We examined the effects of exposure to mild hyperbaric oxygen (MHO) at an atmospheric pressure of 1317 hPa with 40% oxygen on DSS-induced diarrhea and colonic inflammation in rats. Methods Five-week-old male Kyoto Apc Delta (KAD) rats (n = 12) were administered 2% DSS through drinking water for 1 week. Subsequently, DSS-treated male rats were not subjected to any further treatment (n = 6) or exposed to MHO (n = 6) for 2 weeks. Age-matched KAD rats not subjected to DSS treatment or exposed to MHO were used as the control group (n = 6). Results Control rats did not exhibit diarrhea and colonic inflammation. However, DSS-treated rats exhibited diarrhea and colonic inflammation, regardless of exposure to MHO. Exposure to MHO for 2 weeks led to decreased incidence of diarrhea in DSS-treated rats (p < 0.05). Exposure to MHO had no effect on colonic inflammation in DSS-treated rats (p = 0.12). Conclusion Exposure to MHO for 2 weeks can improve diarrhea but cannot attenuate colonic inflammation, possibly due to the short exposure duration (2 weeks) used in this study.