Odd-skipped related 2 splicing variants show opposite transcriptional activity
Odd-skipped related 2 splicing variants show opposite transcriptional activity
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DOI：
10.1016/j.bbrc.2004.12.173
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发表时间：
2005-03-04
影响因子：
3.1
通讯作者：
Amano, A
中科院分区：
文献类型：
--
作者：
Kawai, S;Kato, T;Amano, A
奇数跳跃相关 2 (Osr2) 在牙齿和肾脏发育过程中的上皮-间质相互作用领域表达。在这项研究中，我们报告外显子 4 的选择性剪接产生了两个转录本：Osr2A 和 Osr2B。这些变体利用不同的剪接受体，Osr2B 表现出移码，随后出现早期终止密码子。 Osr2A mRNA 产生的蛋白质在 C 端比 Osr2B 长 36 个氨基酸，并具有 5 个锌指结构域，而 Osr2B 具有 3 个锌指基序。在肾脏、骨骼肌和睾丸中发现了 2 个 Osr2 变体，并且 Osr2B 主要在每个变体中转录。 Flag 标记的变体在细胞核中显示出相同的定位，然而，Ga14 融合蛋白显示出相反的转录活性。此外，Flag 标记的变体也表现出相反的转录活性，尽管它们与 Ga14 融合变体的发现相反。我们的结果表明 Osr2 通过选择性剪接使其功能分叉。 (C) 2005 Elsevier Inc. 保留所有权利。
Odd-skipped related 2 (Osr2) is expressed in the domain of epithelial-mesenchymal interactions during tooth and kidney development. In this study, we report that alternative splicing of exon 4 resulted in two transcripts, Osr2A and Osr2B. These variants utilized a different splice acceptor, and Osr2B exhibited a frameshift followed by an early stop codon. Osr2A mRNA produced a protein that was 36 amino acids longer than Osr2B at the C-terminus and had five zinc-finger domains, whereas Osr2B had three zinc-finger motives. The 2 Osr2 variants were found in kidney, skeletal muscle, and testis, and Osr2B was predominantly transcribed in each. Flag-tagged variants showed the same localization in cell nuclei, however, Ga14 fusion proteins showed opposite transcriptional activities. Further, Flag-tagged variants also showed opposite transcriptional activities, though they were in contrast to the findings for the Ga14-fused variants. Our results indicate that Osr2 bifurcates its function by alternative splicing. (C) 2005 Elsevier Inc. All rights reserved.